caa a22 4500 465765653 CHVBK 20180323111913.0 cr unu---uuuuu 170327e19900901xx s 000 0 eng 10.1007/BF02537169 doi (NATIONALLICENCE)springer-10.1007/BF02537169 Inevitable generation of primary alcohols during reduction of oxidized lipids with sodium borohydride [Elektronische Daten] [Takashi Nakamura, Hiroyuki Maeda, Yoshino Takahashi, Yoichiro Hama] This report deals with the fluorometric determination of fatty alcohols generated by the reduction of the ester linkage of lipids with NaBH4, and with the limitations of the reduction method for assaying oxidized lipids. Optimum conditions for the fluorometric analysis of primary and secondary alcohols using 1-anthroyl nitrile were obtained. After reduction with NaBH4 in MeOH or in MeOH/benzene (8∶2, v/v), the formation of 1-hexadecanol from a variety of palmitic acid esters was measured fluorometrically by reverse-phase high-performance liquid chromatography (HPLC): From glycerides and methyl palmitate, 1-3% (w/w) 1-hexadecanol was produced and a trace was produced from cholesteryl palmitate (10 min, 21°C). 1-Hexadecanol was never generated from palmitic acid. Although considerable improvement occurred with the choice of the solvent for the NaBH4 reduction, the generation of primary alcohols from ester lipids usually seems inevitable. American Oil Chemists' Society, 1990 Nakamura Takashi Laboratory of Fisheries Technology, Faculty of Agriculture, Kyushu University, 6-10-1, Hakozaki, 812, Fukuoka, Japan aut Maeda Hiroyuki Laboratory of Fisheries Technology, Faculty of Agriculture, Kyushu University, 6-10-1, Hakozaki, 812, Fukuoka, Japan aut Takahashi Yoshino Laboratory of Fisheries Technology, Faculty of Agriculture, Kyushu University, 6-10-1, Hakozaki, 812, Fukuoka, Japan aut Hama Yoichiro Laboratory of Fisheries Technology, Faculty of Agriculture, Kyushu University, 6-10-1, Hakozaki, 812, Fukuoka, Japan aut Lipids Springer-Verlag 25/9(1990-09-01), 578-580 0024-4201 25:9<578 1990 25 11745 https://doi.org/10.1007/BF02537169 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02537169 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakamura Takashi Laboratory of Fisheries Technology, Faculty of Agriculture, Kyushu University, 6-10-1, Hakozaki, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Maeda Hiroyuki Laboratory of Fisheries Technology, Faculty of Agriculture, Kyushu University, 6-10-1, Hakozaki, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Takahashi Yoshino Laboratory of Fisheries Technology, Faculty of Agriculture, Kyushu University, 6-10-1, Hakozaki, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Hama Yoichiro Laboratory of Fisheries Technology, Faculty of Agriculture, Kyushu University, 6-10-1, Hakozaki, 812, Fukuoka, Japan aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/9(1990-09-01), 578-580 0024-4201 25:9<578 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer