caa a22 4500 465765696 CHVBK 20180323111913.0 cr unu---uuuuu 170327e19900901xx s 000 0 eng 10.1007/BF02537159 doi (NATIONALLICENCE)springer-10.1007/BF02537159 Exchange of free cholesterol between plasma and erythrocytes from hyperthyroid and hypothyroid rats in Vitro [Elektronische Daten] [F. Ruggiero, F. Cafagna, E. Quagliariello] In our previous studies, we found that circulating thyroid hormone levels alter cholesterol partition between plasma and erythrocytes by changing the phospholipid content of erythrocytes (Ruggiero, F.M.,et al. (1984)Horm. Metabol. Res. 16, 37-40; Ruggiero, F.M.,et al. (1987)Lipids 22, 148-151). As an extension of this work, we now followed the exchange of free cholesterol between plasma and erythrocytes in control, hyperthyroid and hypothyroid rats under various experimental conditionsin vitro. In control rats, erythrocytes incubated with plasma at 37°C for 4 hr lose 10% of cholesterol which was esterified by lecithin: cholesterol acyltransferase (LCAT) present in the plasma. In hyperthyroid rats, erythrocytes incubated with plasma lose 30% of cholesterol within the same time. By contrast, in the case of hypothyroid rats incubation for 4 hr was necessary to transfer 24% of free cholesterol from plasma to erythrocytes. Inhibition of cholesterol esterification did not affect the loss of erythrocyte cholesterol in control and in hyperthyroid rats. Ca2+ increased the LCAT activity in the plasma of these rats. The findings shed light on the role of thyroid hormones in regulating cholesterol levels in plama through active cholesterol transfer between plasma and erythrocytes. American Oil Chemists' Society, 1990 Ruggiero F. Department of Biochemistry and Molecular Biology and C.N.R. Unit for the Study of Mitochondria and Bioenergetics, University of Barl, Via G. Amendola 165/A, 70126, Bari, Italy aut Cafagna F. Department of Biochemistry and Molecular Biology and C.N.R. Unit for the Study of Mitochondria and Bioenergetics, University of Barl, Via G. Amendola 165/A, 70126, Bari, Italy aut Quagliariello E. Department of Biochemistry and Molecular Biology and C.N.R. Unit for the Study of Mitochondria and Bioenergetics, University of Barl, Via G. Amendola 165/A, 70126, Bari, Italy aut Lipids Springer-Verlag 25/9(1990-09-01), 529-533 0024-4201 25:9<529 1990 25 11745 https://doi.org/10.1007/BF02537159 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02537159 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ruggiero F. Department of Biochemistry and Molecular Biology and C.N.R. Unit for the Study of Mitochondria and Bioenergetics, University of Barl, Via G. Amendola 165/A, 70126, Bari, Italy aut NATIONALLICENCE 700 1- Cafagna F. Department of Biochemistry and Molecular Biology and C.N.R. Unit for the Study of Mitochondria and Bioenergetics, University of Barl, Via G. Amendola 165/A, 70126, Bari, Italy aut NATIONALLICENCE 700 1- Quagliariello E. Department of Biochemistry and Molecular Biology and C.N.R. Unit for the Study of Mitochondria and Bioenergetics, University of Barl, Via G. Amendola 165/A, 70126, Bari, Italy aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/9(1990-09-01), 529-533 0024-4201 25:9<529 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer