caa a22 4500 465765742 CHVBK 20180323111913.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF02537985 doi (NATIONALLICENCE)springer-10.1007/BF02537985 Quantitation of vitamin K in human milk [Elektronische Daten] [Louise Canfield, Judy Hopkinson, Anne Lima, Gail Martin, Kyoto Sugimoto, Jeanne Burr, Larry Clark, Daniel McGee] A quantitative method was developed for the assay of vitamin K in human colostrum and milk. The procedure combines preparative and analytical chromatography on silica gel in a nitrogen atmosphere followed by reversed phase high performance liquid chromatography (HPLC). Two HPLC steps were used: gradient separation with ultraviolet (UV) detection followed by isocratic separation detected electrochemically. Due to co-migrating impurities, UV detection alone is insufficient for identification of vitamin K. Exogenous vitamin K was shown to equilibrate with endogenous vitamin K in the samples. A statistical method was incorporated to control for experimental variability. Vitamin K1 was analyzed in 16 pooled milk samples from 7 donors and in individual samples from 15 donors at 1 month post-partrum. Vitamin K1 was present at 2.94±1.94 and 3.15±2.87 ng/mL in pools and in individuals, respectively. Menaquinones, the bacterial form of the vitamin, were not detected. The significance of experimental variation to studies of vitamin K in individuals is discussed. American Oil Chemists' Society, 1990 Canfield Louise Department of Biochemistry and Family and Community Medicine, University of Arizona, 85721, Tucson, Arizona aut Hopkinson Judy Department of Pediatrics, Baylor College of Medicine, 77030, Houston, Texas aut Lima Anne Process Development Department, Pitman-Moore, P.O. Box 207, 47808, Terre Haute, IN aut Martin Gail Department of Biochemistry, Texas A&M University, 77843, College Station, TX aut Sugimoto Kyoto 11731 Wickhollow Lane, 77043, Houston, TX aut Burr Jeanne Department of Biochemistry and Family and Community Medicine, University of Arizona, 85721, Tucson, Arizona aut Clark Larry Department of Biochemistry and Family and Community Medicine, University of Arizona, 85721, Tucson, Arizona aut McGee Daniel Department of Biochemistry and Family and Community Medicine, University of Arizona, 85721, Tucson, Arizona aut Lipids Springer-Verlag 25/7(1990-07-01), 406-411 0024-4201 25:7<406 1990 25 11745 https://doi.org/10.1007/BF02537985 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02537985 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Canfield Louise Department of Biochemistry and Family and Community Medicine, University of Arizona, 85721, Tucson, Arizona aut NATIONALLICENCE 700 1- Hopkinson Judy Department of Pediatrics, Baylor College of Medicine, 77030, Houston, Texas aut NATIONALLICENCE 700 1- Lima Anne Process Development Department, Pitman-Moore, P.O. Box 207, 47808, Terre Haute, IN aut NATIONALLICENCE 700 1- Martin Gail Department of Biochemistry, Texas A&M University, 77843, College Station, TX aut NATIONALLICENCE 700 1- Sugimoto Kyoto 11731 Wickhollow Lane, 77043, Houston, TX aut NATIONALLICENCE 700 1- Burr Jeanne Department of Biochemistry and Family and Community Medicine, University of Arizona, 85721, Tucson, Arizona aut NATIONALLICENCE 700 1- Clark Larry Department of Biochemistry and Family and Community Medicine, University of Arizona, 85721, Tucson, Arizona aut NATIONALLICENCE 700 1- McGee Daniel Department of Biochemistry and Family and Community Medicine, University of Arizona, 85721, Tucson, Arizona aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/7(1990-07-01), 406-411 0024-4201 25:7<406 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer