caa a22 4500 465765769 CHVBK 20180323111913.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF02537979 doi (NATIONALLICENCE)springer-10.1007/BF02537979 Palmitic acid-labeled lipids selectively incorporated into platelet cytoskeleton during aggregation [Elektronische Daten] [M. Packham, M. Guccione, N. Bryant, A. Livne] Previous experiments showed that during the early stages (20-30 seconds) of aggregation induced by adenosine diphosphate (ADP, 2 μM) or thrombin (0.1 U/mL) of rabbit or human platelets prelabeled with [3H]palmitic acid, labeled lipid became associated with the cytoskeleton isolated after lysis with 1% Triton X-100, 5 mM EGTA [ethylene glycol-bis-(β-aminoethyl ether)]-N,N,N',N'-tetra-acetic acid. The association appeared to be related to the number of sites of contact and was independent of the release of granule contents. We have now investigated the nature of the labeled lipids by thin-layer and column chromatography and found differences between the distribution of the label in intact platelets (both stimulated and unstimulated) and the isolated cytoskeletons. In both species and with either ADP or thrombin as aggregating agent, 70-85% of the label in both intact platelets and in the cytoskeletons was in phospholipids. The distribution of label among the phospholipids in the cytoskeletons was similar to that in intact platelets except that the percentage of label in phosphatidylcholine was significantly higher in the cytoskeletons of human platelets than in the intact platelets, and the percentage of label in phosphatidylserine/phosphatidylinositol was significantly lower in the cytoskeletons of rabbit platelets and thrombin-aggregated human platelets than in intact platelets. The cytoskeletons contained a lower percentage of label in triacylglycerol, diacylglycerol, and cholesterol ester than the intact platelets. Contrary to a report in the literature, we found no evidence for the incorporation of diacylglycerol and palmitic acid into the cytoskeleton. Although intact rabbit platelets had more label in ceramide (6.7±2.9%) than intact human platelets (1.5±0.9%), platelets of both species exhibited a three- to four-fold enrichment of labeled ceramide in the cytoskeletons. Thus phospholipids and ceramide that are readily labeled with palmitic acid are selectively incorporated into the cytoskeleton during the initial stages of platelet aggregation. American Oil Chemists' Society, 1990 Packham M. Department of Biology, ben-Gurion University of the Negev, Beersheva, Israel aut Guccione M. Department of Biochemistry, University of Toronto, M5S 1A8, Toronto, Ontario, Canada aut Bryant N. Department of Biochemistry, University of Toronto, M5S 1A8, Toronto, Ontario, Canada aut Livne A. Department of Biochemistry, University of Toronto, M5S 1A8, Toronto, Ontario, Canada aut Lipids Springer-Verlag 25/7(1990-07-01), 371-378 0024-4201 25:7<371 1990 25 11745 https://doi.org/10.1007/BF02537979 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02537979 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Packham M. Department of Biology, ben-Gurion University of the Negev, Beersheva, Israel aut NATIONALLICENCE 700 1- Guccione M. Department of Biochemistry, University of Toronto, M5S 1A8, Toronto, Ontario, Canada aut NATIONALLICENCE 700 1- Bryant N. Department of Biochemistry, University of Toronto, M5S 1A8, Toronto, Ontario, Canada aut NATIONALLICENCE 700 1- Livne A. Department of Biochemistry, University of Toronto, M5S 1A8, Toronto, Ontario, Canada aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/7(1990-07-01), 371-378 0024-4201 25:7<371 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer