caa a22 4500 465765777 CHVBK 20180323111913.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF02537987 doi (NATIONALLICENCE)springer-10.1007/BF02537987 Separation and detection of phospholipid hydroperoxides in the low nanomolar range by a high performance liquid chromatography/irothiocyanate assay [Elektronische Daten] [Anette Mullertz, Anne Schmedes, Gunhild Hølmer] A new method for the detection of phospholipid hydroperoxides in the low nanomolar range has been developed by using a high performance liquid chromatography system combined with a post-column reaction. The detection is based on the oxidation of Fe(II) to Fe(III) by hydroperoxides and the subsequent formation of an ironthiocyanate complex, which can be determined spectrophotometrically at 505 nm. By this method it is possible to separate and quantitate phosphatidylethanolamine hydroperoxide and phosphatidylcholine hydroperoxide formed in erythrocyte ghost membranes during photo-oxidation. American Oil Chemists' Society, 1990 Mullertz Anette Department of Biochemistry and Nutrition, Technical University of Denmark, Building 224, DK-2800, Lyngby, Denmark aut Schmedes Anne Department of Biochemistry and Nutrition, Technical University of Denmark, Building 224, DK-2800, Lyngby, Denmark aut Hølmer Gunhild Department of Biochemistry and Nutrition, Technical University of Denmark, Building 224, DK-2800, Lyngby, Denmark aut Lipids Springer-Verlag 25/7(1990-07-01), 415-418 0024-4201 25:7<415 1990 25 11745 https://doi.org/10.1007/BF02537987 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02537987 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mullertz Anette Department of Biochemistry and Nutrition, Technical University of Denmark, Building 224, DK-2800, Lyngby, Denmark aut NATIONALLICENCE 700 1- Schmedes Anne Department of Biochemistry and Nutrition, Technical University of Denmark, Building 224, DK-2800, Lyngby, Denmark aut NATIONALLICENCE 700 1- Hølmer Gunhild Department of Biochemistry and Nutrition, Technical University of Denmark, Building 224, DK-2800, Lyngby, Denmark aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/7(1990-07-01), 415-418 0024-4201 25:7<415 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer