caa a22 4500 465765785 CHVBK 20180323111913.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF02537980 doi (NATIONALLICENCE)springer-10.1007/BF02537980 Effect of excess dietary calcium on colon mucosal membranes and fecal lipids [Elektronische Daten] [Atif Awad, Mary Short, Carol Fink] The objective of the present studies was to examine the biochemical alterations in colon apical membranes upon feeding excess calcium to animals on a saturated fatty acid-rich diet. It has been suggested recently that excess dietary calcium may offer a protection to colon membranes as judged by histologic examination. Sprague-Dawley weanling male rats were fed a semisynthetic diet containing 14% beef fat plus 2% corn oil and either the calcium requirement or excess calcium in the form of calcium carbonate. Animals were fed the diets for 4 weeks. Feces were collected in the last 3 days. The results indicate that excess dietary calcium resulted in alteration in the density of 4 protein bands of colon apical membranes upon examination on SDS-gel electrophoresis. These bands contain 20% of membrane proteins. The diet had no effect on either the lipid content or fatty acid composition of the membranes. Excess dietary calcium resulted in 54% reduction in fecal water bile acids and a 44% reduction in fecal water free fatty acids. The reduction in fecal water lipids was due to alterations in the solubility of these lipids. This was not mediated through alterations in the pH of fecal water. The observed alterations in protein patterns of these membranes may be due to either the reduction of fecal water bile acids and free fatty acids or may be a direct effect of dietary calcium on membrane proteins. American Oil Chemists' Society, 1990 Awad Atif Nutrition Program, State University of New York at Buffalo, 323 Kimball Tower, SUN, 14214, Buffalo, NY aut Short Mary Nutrition Program, State University of New York at Buffalo, 323 Kimball Tower, SUN, 14214, Buffalo, NY aut Fink Carol Nutrition Program, State University of New York at Buffalo, 323 Kimball Tower, SUN, 14214, Buffalo, NY aut Lipids Springer-Verlag 25/7(1990-07-01), 379-382 0024-4201 25:7<379 1990 25 11745 https://doi.org/10.1007/BF02537980 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02537980 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Awad Atif Nutrition Program, State University of New York at Buffalo, 323 Kimball Tower, SUN, 14214, Buffalo, NY aut NATIONALLICENCE 700 1- Short Mary Nutrition Program, State University of New York at Buffalo, 323 Kimball Tower, SUN, 14214, Buffalo, NY aut NATIONALLICENCE 700 1- Fink Carol Nutrition Program, State University of New York at Buffalo, 323 Kimball Tower, SUN, 14214, Buffalo, NY aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/7(1990-07-01), 379-382 0024-4201 25:7<379 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer