caa a22 4500 465765815 CHVBK 20180323111913.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF02537981 doi (NATIONALLICENCE)springer-10.1007/BF02537981 Cell separation of Tethya aurantia , an analytical study of embryonic and differentiated sponge cells [Elektronische Daten] [Mary Zimmerman, Max Hoberg, Eser Ayanoglu, Carl Djerassi] The cells of the spongeTethya aurantia var.californiana were separated on a Ficoll density gradient and the fractions analyzed for cell types and their lipids. Major cell types were choanocyte, archeocyte, and symbiont. Major differences in archeocyte and choanocyte fatty acid composition were noted for 20∶4, 26∶1 and 26∶2. The fatty acids 26∶1, 26∶2, and 28∶3 were dominant in the phosphatidylcholine fraction. Archeocytes had highest concentrations of 4,7,10,13-20∶4 and 5,8,11,14-20∶4 (arachidonic) acids which could be derived from symbionts, as odd-chain and methyl-branched fatty acid were also present. Sterol analyses showed cholesterol as a major sterol of the sponge cell fractions and clionasterol (or its 24-isomer) as a major sterol in symbiont cells. American Oil Chemists' Society, 1990 Zimmerman Mary Department of Chemistry, Stanford University, 94305-5080, Stanford, CA aut Hoberg Max Department of Chemistry, Stanford University, 94305-5080, Stanford, CA aut Ayanoglu Eser Department of Chemistry, Stanford University, 94305-5080, Stanford, CA aut Djerassi Carl Department of Chemistry, Stanford University, 94305-5080, Stanford, CA aut Lipids Springer-Verlag 25/7(1990-07-01), 383-390 0024-4201 25:7<383 1990 25 11745 https://doi.org/10.1007/BF02537981 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02537981 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zimmerman Mary Department of Chemistry, Stanford University, 94305-5080, Stanford, CA aut NATIONALLICENCE 700 1- Hoberg Max Department of Chemistry, Stanford University, 94305-5080, Stanford, CA aut NATIONALLICENCE 700 1- Ayanoglu Eser Department of Chemistry, Stanford University, 94305-5080, Stanford, CA aut NATIONALLICENCE 700 1- Djerassi Carl Department of Chemistry, Stanford University, 94305-5080, Stanford, CA aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/7(1990-07-01), 383-390 0024-4201 25:7<383 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer