caa a22 4500 465765831 CHVBK 20180323111913.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF02537984 doi (NATIONALLICENCE)springer-10.1007/BF02537984 Measurement of the metabolic interconversion of deuterium-labeled fatty acids by gas chromatography/mass spectrometry [Elektronische Daten] [William Rohwedder, Sandra Duval, Darhal Wolf, Edward Emken] An analytical method that was developed to analyze deuterium-labeled fatty acids in human blood has been extended to identify labeled fatty acids from C14 to C24 chain length which are formed by metabolic processes such as desaturation, elongation, or shortening of the labeled fatty acids fed. A new computer and a hard-wave adder have been utilized to assure reliable data acquisition. Relative standard derivations for the analysis of labeled fatty acids were measured at 0.02, 0.03, and 0.04 at the 5%, 1%, and 0.2% levels of the labeled fatty acid methyl esters, respectively. The method makes extensive use of standards and computer processing for accuracy and high productivity. Data from a chylomicron triacylglycerol fraction are included to demonstrate the sensitivity of detection of metabolites formed by desaturation and elongation. American Oil Chemists' Society, 1990 Rohwedder William Vegetable Oil Research, Northern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 1815 N. University Street, 61604, Peoria, Illinois aut Duval Sandra Vegetable Oil Research, Northern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 1815 N. University Street, 61604, Peoria, Illinois aut Wolf Darhal Vegetable Oil Research, Northern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 1815 N. University Street, 61604, Peoria, Illinois aut Emken Edward Vegetable Oil Research, Northern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 1815 N. University Street, 61604, Peoria, Illinois aut Lipids Springer-Verlag 25/7(1990-07-01), 401-405 0024-4201 25:7<401 1990 25 11745 https://doi.org/10.1007/BF02537984 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02537984 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rohwedder William Vegetable Oil Research, Northern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 1815 N. University Street, 61604, Peoria, Illinois aut NATIONALLICENCE 700 1- Duval Sandra Vegetable Oil Research, Northern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 1815 N. University Street, 61604, Peoria, Illinois aut NATIONALLICENCE 700 1- Wolf Darhal Vegetable Oil Research, Northern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 1815 N. University Street, 61604, Peoria, Illinois aut NATIONALLICENCE 700 1- Emken Edward Vegetable Oil Research, Northern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 1815 N. University Street, 61604, Peoria, Illinois aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/7(1990-07-01), 401-405 0024-4201 25:7<401 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer