caa a22 4500 46576584X CHVBK 20180323111913.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF02537977 doi (NATIONALLICENCE)springer-10.1007/BF02537977 Mechanism of lysophosphatidylcholine accumulation in the ischemic canine heart [Elektronische Daten] [Thomas Mock, Ricky Man] The metabolism of lysophosphatidylcholine (LPC) in non-ischemic and ischemic canine heart was investigated byin vitro enzyme analysis. Selected subcellular fractions were assayed for the LPC-producing enzyme phospholipase A and the LPC-eliminating enzymes LPC:acyl-CoA acyltransferase, LPC:LPC transacylase and lysophospholipase. The canine heart was found to contain all enzymes differing, however, in subcellular distribution and specific activity. Phospholipase A activity did not change significantly in any of the fractions prepared from the ischemic tissue of hearts rendered ischemic for 1, 3 or 5 hr when compared to non-ischemic tissue. Changes in the activity of the microsomal LPC:acyl-CoA acyltransferase over the course of 5 hr of ischemia were observed. Significant decreases in the activity of the cytosolic and microsomal lysophospholipases were detected especially after 3 and 5 hr of ischemia. Similarly, a decrease in the activity of the microsomal LPC:LPC transacylase was noted after 3 and 5 hr of ischemia. Our results suggest that impaired catabolism of LPC rather than an enhanced production of LPC is the principal mechanism for the increase in LPC levels in the ischemic canine heart. American Oil Chemists' Society, 1990 Mock Thomas Department of Pharmacology and Therapeutics, University of Manitoba, Faculty of Medicine, 770 Bannatyne Ave., R3E OW3, Winnipeg, Manitoba, Canada aut Man Ricky Department of Pharmacology and Therapeutics, University of Manitoba, Faculty of Medicine, 770 Bannatyne Ave., R3E OW3, Winnipeg, Manitoba, Canada aut Lipids Springer-Verlag 25/7(1990-07-01), 357-362 0024-4201 25:7<357 1990 25 11745 https://doi.org/10.1007/BF02537977 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02537977 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mock Thomas Department of Pharmacology and Therapeutics, University of Manitoba, Faculty of Medicine, 770 Bannatyne Ave., R3E OW3, Winnipeg, Manitoba, Canada aut NATIONALLICENCE 700 1- Man Ricky Department of Pharmacology and Therapeutics, University of Manitoba, Faculty of Medicine, 770 Bannatyne Ave., R3E OW3, Winnipeg, Manitoba, Canada aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/7(1990-07-01), 357-362 0024-4201 25:7<357 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer