caa a22 4500 465765874 CHVBK 20180323111913.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF02535907 doi (NATIONALLICENCE)springer-10.1007/BF02535907 Baillargeon Mary Agricultural Research Service, Eastern Regional Research Center, U.S. Department of Agricuture, 600 East Mermaid Lane, 19118, Philadelphia, PA aut Purification and specificity of lipases from Geotrichum candidum [Elektronische Daten] [Mary Baillargeon] A crude, commercialGeotrichum candidum lipase (EC 3.1.1.3) preparation (Amano GC-20) was purified by hydrophobic interaction chromatography on Octyl Sepharose. The purified enzyme is a microheterogeneous glycoprotein containing isozymes varying in molecular weight, pI and specificity. It consists of 64, 62 and 59 kDa species as determined by denaturing polyacrylamide gel electrophoresis. Five isozymes (pI 4.40, 4.47, 4.58, 4.67 and 4.72) are detected by isoelectric focusing using both silver and activity stains. Chromatofocusing was used to separate the isozymes according to pI. Although all the isozymes are specific for oleatevs stearate esters, one isozyme (pI 4.72) is also specific for oleatevs palmitate. The number of isozymes is reduced to two (pI 4.67 and 4.72) after carbohydrate removal using endoglycosidase F/N-glycosidase. These isozymes may be products of two lipase genes. American Oil Chemists' Society, 1990 Lipids Springer-Verlag 25/12(1990-12-01), 841-848 0024-4201 25:12<841 1990 25 11745 https://doi.org/10.1007/BF02535907 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02535907 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Baillargeon Mary Agricultural Research Service, Eastern Regional Research Center, U.S. Department of Agricuture, 600 East Mermaid Lane, 19118, Philadelphia, PA aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/12(1990-12-01), 841-848 0024-4201 25:12<841 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer