caa a22 4500 465765904 CHVBK 20180323111913.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF02535902 doi (NATIONALLICENCE)springer-10.1007/BF02535902 Influence of dietary fish oil on the relative synthesis of triacylglycerol and phospholipids in rat liver in vivo [Elektronische Daten] [Young Yeo, Bruce Holub] The influence of dietary fish oil containing n−3 polyunsaturated fatty acids on the biosynthesis of triacylglycerol relative to total individual phospholipids was studied in rat liverin vivo. The dietary lipid (10% by weight of diet) was either sunflower oil enriched in linoleic acid (SO group) or MaxEPA fish oil/sunflower oil, 9∶1 by weight (FO group) enriched in eicosapentaenoic acid (EPA, 20∶5n−3) plus docosahexaenoic acid (DHA, 22∶6n−3). After a 3-week feeding period, the triacylglycerol content (in μmmol/g liver) was 44% lower in the FO group relative to the SO animals. Thein vivo incorporation of [3H]glycerol into individual hepatic lipids resulted in triacyl-glycerol/total phospholipid radioactivity ratios of 2.1 and 0.9 for the SO and FO groups, respectively. These results indicate an inhibitory effect of dietary EPA/DHA on triacylglycerol relative to phospholipid synthesis from intermediary 1,2-diacylglycerol in rat liverin vivo. This metabolic alteration was accompanied by a substantially lower amount (in μmol/g liver) of arachidonic acid and higher levels of EPA plus DHA in the triacylglycerol, choline glycerophospholipid (CGP), and ethanolamine glycerophospholipid (EGP) of the FO group. A moderately higher labelling of the EGP from [3H]glycerol was observed in the FO as compared to the SO group (as evidenced by CGP/EGP radioactivity ratios of 1.3∶1 and 1.8∶1, respectively). The present study providesin vivo evidence for a dampening effect of dietary fish oil on the synthesis of liver triacylglycerol relative to phospholipid and a moderate alteration ofde novo synthesis of individual phospholipids. American Oil Chemists' Society, 1990 Yeo Young Department of Dairy Sciences, Kyungpook National University, Taegu, Korea aut Holub Bruce Department of Nutritional Sciences, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut Lipids Springer-Verlag 25/12(1990-12-01), 811-814 0024-4201 25:12<811 1990 25 11745 https://doi.org/10.1007/BF02535902 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02535902 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yeo Young Department of Dairy Sciences, Kyungpook National University, Taegu, Korea aut NATIONALLICENCE 700 1- Holub Bruce Department of Nutritional Sciences, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/12(1990-12-01), 811-814 0024-4201 25:12<811 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer