caa a22 4500 465765920 CHVBK 20180323111913.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF02535910 doi (NATIONALLICENCE)springer-10.1007/BF02535910 Separation of 20∶4n−6 and 20∶4n−7 by capillary gas-liquid chromatography [Elektronische Daten] [Robert Wolff, Jean-Louis Sebedio, Andre Grandgirard] The use of a capillary column coated with 100% cyanopropyl polysiloxane (CPTMSil 88) allows the separation of several fatty acids associated with fat deficiency. Starting with liver mitochondrial phospholipids of weanling rats fed a fat-free diet, an unusual fatty acid was isolated, along with 20∶4n−6, by thin-layer chromatography on AgNO3-impregnated silica gel plates. After partial hydrazine reduction of these acids, the resulting monoenes were isolated and subjected to ozonolysis in BF3/methanol. The resulting monomethyl and dimethyl esters were identified by gas chromatography/mass spectrometry. Our data indicate that the unusual component corresponds to 20∶4n−7. Based on published biochemical and analytical studies and on our own chromatographic retention data, some of the other unusual fatty acids were tentatively identified as 18∶2n−7, 20∶2n−7 and 20∶3n−7. The CPTMSil 88 column appears to be a simple and useful tool for the separation of fatty acids of the palmitoleate series. American Oil Chemists' Society, 1990 Wolff Robert ISTAB-ITERG, Laboratoire de Lipochimie Alimentaire, Universite de Bordeaux I, Alée des Facultés, 33405, Talence Cedex, France aut Sebedio Jean-Louis Station de Recherche sur la Qualite des Aliments de l'Homme, Unite de Nutrition Lipidique, INRA, 21034, Dijon Cedex, France aut Grandgirard Andre aut Lipids Springer-Verlag 25/12(1990-12-01), 859-862 0024-4201 25:12<859 1990 25 11745 https://doi.org/10.1007/BF02535910 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02535910 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wolff Robert ISTAB-ITERG, Laboratoire de Lipochimie Alimentaire, Universite de Bordeaux I, Alée des Facultés, 33405, Talence Cedex, France aut NATIONALLICENCE 700 1- Sebedio Jean-Louis Station de Recherche sur la Qualite des Aliments de l'Homme, Unite de Nutrition Lipidique, INRA, 21034, Dijon Cedex, France aut NATIONALLICENCE 700 1- Grandgirard Andre aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/12(1990-12-01), 859-862 0024-4201 25:12<859 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer