caa a22 4500 46576598X CHVBK 20180323111914.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF02535909 doi (NATIONALLICENCE)springer-10.1007/BF02535909 The metabolism of 20- and 22-carbon unsaturated acids in rat heart and myocytes as mediated by feeding fish oil [Elektronische Daten] [B. Mohammed, Tor-Arne Hagve, Howard Sprecher] When rats were fed 5% corn oil, the heart phospholipids contained large amounts of 22-carbon (n−6) acids. When half of the corn oil was replaced with fish oil, the reduced level of arachidonate and 22-carbon (n−6) acids in phospholipids was accompanied by increases in the levels of 22-carbon (n−3) acids while only small amounts of 20∶5(n−3) were acylated. Heart myocytes readily took up and acylated [1-14C]-labeled 20∶4(n−6), 20∶5(n−3) and 22∶6(n−3) into phospholipids. The uptake and acylation of 20∶4(n−6) was greater than for 20∶5(n−3) but the intracellular labeling profiles were similar. Uptake and acylation of 22∶6(n−3) was somewhat lower. In addition the intracellular labeling profile differed in that more 22∶6(n−3) was incorporated into the ethanolamine-containing phospholipids than when 20∶4(n−6) or 20∶5(n−3) were the substrates. Neither 20∶4(n−6) nor 20∶5(n−3) was chain elongated. When [3-14C]-labeled 22∶4(n−6) and 22∶5(n−3) were the substrates, it was not possible to detect radioactive 22∶5(n−6) or 22∶6(n−3). Both [3-14]-labeled substrates were acylated into phospholipids and retroconverted with the subsequent esterification of radioactive 20∶4(n−6) and 20∶5(n−3) into triglycerides and phospholipids. These studies show that cardiomyocytes lack the ability to make 22-carbon acids from 20-carbon precursors but they retroconvert 22-carbon acids to 20-carbon acids. The high levels of 22-carbon polyunsaturated acids in total heart lipids thus cannot be attributed to the synthetic capacities of cardiomyocytes. American Oil Chemists' Society, 1990 Mohammed B. The Department of Physiological Chemistry, College of Medicine, The Ohio State University, 5170 Graves Hall, 333 W. 10th Avenue, 43210, Columbus, OH aut Hagve Tor-Arne The Department of Physiological Chemistry, College of Medicine, The Ohio State University, 5170 Graves Hall, 333 W. 10th Avenue, 43210, Columbus, OH aut Sprecher Howard The Department of Physiological Chemistry, College of Medicine, The Ohio State University, 5170 Graves Hall, 333 W. 10th Avenue, 43210, Columbus, OH aut Lipids Springer-Verlag 25/12(1990-12-01), 854-858 0024-4201 25:12<854 1990 25 11745 https://doi.org/10.1007/BF02535909 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02535909 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mohammed B. The Department of Physiological Chemistry, College of Medicine, The Ohio State University, 5170 Graves Hall, 333 W. 10th Avenue, 43210, Columbus, OH aut NATIONALLICENCE 700 1- Hagve Tor-Arne The Department of Physiological Chemistry, College of Medicine, The Ohio State University, 5170 Graves Hall, 333 W. 10th Avenue, 43210, Columbus, OH aut NATIONALLICENCE 700 1- Sprecher Howard The Department of Physiological Chemistry, College of Medicine, The Ohio State University, 5170 Graves Hall, 333 W. 10th Avenue, 43210, Columbus, OH aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/12(1990-12-01), 854-858 0024-4201 25:12<854 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer