caa a22 4500 465765998 CHVBK 20180323111914.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF02535908 doi (NATIONALLICENCE)springer-10.1007/BF02535908 Formation of 8,15-dihydroxy eicosatetraenoic acid via 15- and 12-lipoxygenases in fish gill [Elektronische Daten] [J. German, Ralf Berger] Metabolites of arachidonic acid are important regulators of biological function in a variety of mammalian tissues. We have demonstrated similar lipoxygenase enzyme activities in fish gills and mammalian lungs suggesting that their metabolites may have matching functions. Fish gills were investigated for their ability to generate dioxygenated metabolites of polyunsaturated fatty acids. Fatty acids, including arachidonic acid, were incubated with crude tissue homogenates and polar metabolites were extracted, derivatized and analyzed by high performance liquid chromatography, gas chromatography and mass spectrometry. The major dihydroxy metabolite of arachidonic acid was characterized as 8(LR), 15(LS)-dihydroxyeicosatetraenoic acid. This product was formed by the sequential action of the 15- and 12-lipoxygenases in the tissue. The formation of the dihydroxyeicosatetraenoic acid by crude tissue homogenates was significantly enhanced by the addition of 1 mM reduced glutathione. The metabolism of other polyunsaturated fatty acids, including eicosapentaenoic acid and docosahexaenoic acid, to dihydroxy acids was consistent with their relative ability to serve as substrates for the initial 15-lipoxygenase reaction. American Oil Chemists' Society, 1990 German J. Department of Food Science and Technology, University of California, Davis, 95616, Davis, California aut Berger Ralf Institut fur Lebensmitteltechnologie und Analytische Chemie der Technischen Universität München, D-8050, Freising 12, Federal Republic of Germany aut Lipids Springer-Verlag 25/12(1990-12-01), 849-853 0024-4201 25:12<849 1990 25 11745 https://doi.org/10.1007/BF02535908 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02535908 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- German J. Department of Food Science and Technology, University of California, Davis, 95616, Davis, California aut NATIONALLICENCE 700 1- Berger Ralf Institut fur Lebensmitteltechnologie und Analytische Chemie der Technischen Universität München, D-8050, Freising 12, Federal Republic of Germany aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/12(1990-12-01), 849-853 0024-4201 25:12<849 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer