caa a22 4500 465766048 CHVBK 20180323111914.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF02544332 doi (NATIONALLICENCE)springer-10.1007/BF02544332 Albumin-bound docosahexaenoic acid and collagen-induced human platelet reactivity [Elektronische Daten] [Douglas Gaudette, Bruce Holub] Anin vitro system designed to mimic the effect of various plasma nonesterified (polyunsaturated) fatty acids on platelet function and metabolism was employed. Human platelet aggregation induced by submaximal (1.8 μg/ml) collagen stimulation was significantly inhibited by 2 min preincubation with 20 μM albumin-bound docosahexaenoic acid (22∶6n−3) (DHA), but not by the other fatty acids tested. [3H]Phosphatidic acid (PA) formation, an indicator of phospholipase C activation following platelet stimulation, was moderately inhibited by eicosapentaenoic acid (20∶5n−3), 11,14,17-eicosatrienoic acid (20∶3n−3), dihomo-γ-linolenic acid (20∶3n−6), as well as DHA, but not by arachidonic acid (20∶4n−6); this inhibition of phospholipase C activation could not explain the differential effect of DHA on platelet aggregation. The decreased production of thromboxane A2 (TxA2), as assessed by [3H]12-hydroxy-5,8,10-heptadecatrienoic acid (HHT) formation, may account for the inhibition of collagen-induced aggregation by 20 μM DHA. Surprisingly, preincubation with 40 μM albumin-bound DHA, even though resulting in greater inhibition of collagen-induced aggregation, had less impact on HHT formation. A small but significant increase in [3H]prostaglandin D2 (PGD2) levels following 3-min collagen stimulation may have contributed to the greater antiaggregatory effect of 40 μM DHA. It is concluded that increased plasma nonesterified DHA may contribute to the dampened platelet activation and altered metabolism following fish oil supplementation of the diet. American Oil Chemists' Society (AOCS), 1985 Gaudette Douglas Department of Nutritional Sciences, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut Holub Bruce Department of Nutritional Sciences, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut Lipids Springer-Verlag 25/3(1990-03-01), 166-169 0024-4201 25:3<166 1990 25 11745 https://doi.org/10.1007/BF02544332 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02544332 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gaudette Douglas Department of Nutritional Sciences, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut NATIONALLICENCE 700 1- Holub Bruce Department of Nutritional Sciences, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/3(1990-03-01), 166-169 0024-4201 25:3<166 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer