caa a22 4500 465766145 CHVBK 20180323111914.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF02536018 doi (NATIONALLICENCE)springer-10.1007/BF02536018 A fluorimetric method for measuring lipase activity based on umbelliferyl esters [Elektronische Daten] [Thierry de Monpezat, Bernard de Jeso, Jean-Luc Butour, Louis Chavant, Michel Sancholle] A new fluorimetric method for measuring lipase activity is described. The fluorescent compound umbelliferone (UMB) was esterified with various unsaturated and saturated fatty acids of different carbon chain lengths, and the non-fluorescent esters were used as substrates for measuring the activity of two lipases purified fromRhizopus delemar andCandida cylindracea. The fluorescence intensity of UMB liberated by hydrolysis of the substrates gives an indication of the lipase activity towards the respective esters. UMB was found to be somewhat more sensitive and stable than 4-methylumbelliferone (4-MU), which has been used previously. The highly sensitive method (nmoles UMB/mL/hr) described here was tested on five different esters. The method has the advantage of not requiring extraction of the lipolysis products. It also can be carried out quickly, in two to three minutes, and thus lends itself to the screening of lipase-producing micro-organisms. The method offers an interesting alternative or can be complementary to assays based on the use of 4-methylumbelliferone esters as substrates. American Oil Chemists' Society, 1990 de Monpezat Thierry Laboratoire de Cryptogamie, 31O62, Toulouse Cedex aut de Jeso Bernard Laboratoire de Chimie Organique et Organométallique (U.A.35 CNRS), 33O45, Talence Cedex aut Butour Jean-Luc Laboratoire de Pharmacologie et Toxicologie Fondamentales, CNRS, 31O62, Toulouse Cedex aut Chavant Louis Laboratoire de Cryptogamie, 31O62, Toulouse Cedex aut Sancholle Michel Laboratoire de Cryptogamie, Universite des Sciences et Techniques de Lille, 59655, Villeneuve d'Ascq Cedex, France aut Lipids Springer-Verlag 25/10(1990-10-01), 661-664 0024-4201 25:10<661 1990 25 11745 https://doi.org/10.1007/BF02536018 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02536018 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- de Monpezat Thierry Laboratoire de Cryptogamie, 31O62, Toulouse Cedex aut NATIONALLICENCE 700 1- de Jeso Bernard Laboratoire de Chimie Organique et Organométallique (U.A.35 CNRS), 33O45, Talence Cedex aut NATIONALLICENCE 700 1- Butour Jean-Luc Laboratoire de Pharmacologie et Toxicologie Fondamentales, CNRS, 31O62, Toulouse Cedex aut NATIONALLICENCE 700 1- Chavant Louis Laboratoire de Cryptogamie, 31O62, Toulouse Cedex aut NATIONALLICENCE 700 1- Sancholle Michel Laboratoire de Cryptogamie, Universite des Sciences et Techniques de Lille, 59655, Villeneuve d'Ascq Cedex, France aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/10(1990-10-01), 661-664 0024-4201 25:10<661 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer