caa a22 4500 465766161 CHVBK 20180323111914.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF02536017 doi (NATIONALLICENCE)springer-10.1007/BF02536017 Metabolic fate of sphingomyelin of high-density lipoprotein in rat plasma [Elektronische Daten] [Marc Bentejac, Maurice Bugaut, Marie Delachambre, Jean Lecerf] The metabolic fate of high density lipoprotein (HDL) sphingomyelin in plasma was studied in rats over a 24-hr period after injection of HDL containing sphingomyelin which was14C-labeled in the stearic (18∶0) or lignoceric acid (24∶0) moiety and3H-labeled in the choline methyl groups. Decay of label in plasma followed three phases. The first two phases were similar for both isotopes and both types of sphingomyelin (t1/2≃10 and 110 min). However, during the third phase (from 10 hr after injection),3H label disappeared more slowly than14C label from 18∶0 sphingomyelin, whereas the3H/14C ratio remained relatively constant when 24∶0 sphingomyelin was used. Intact, doubly-labeled 18∶0 sphingomyelin disappeared from HDL rapidly (t1/2=38 min) by tissue uptake and by transfer to very low density lipoprotein (VLDL). VLDL contained up to 12% of the sphingomyelin 1 hr after injection. This is the first demonstration of a transferin vivo of sphingomyelin from HDL to VLDL. A similarly rapid transfer was also observedin vitro. Some nontritated, [14C]18∶0 or [14C]24∶0 sphingomyelin was redistributed more slowly into HDL. Doubly-labeled phosphatidylcholine appeared in VLDL and HDL within 1 hr after injection and reached 1.8 and 2.1% of the injected14C and3H in VLDL at 1 hr, and 4.8 and 6.9% in HDL at 3 hr, respectively. American Oil Chemists' Society, 1990 Bentejac Marc Laboratoire de Physiologie Animale et de la Nutrition, Faculte des Sciences Mirande, Universite de Dijon, BP 138, 21004, Dijon Cedex aut Bugaut Maurice Laboratoire de Physiologie Animale et de la Nutrition, Faculte des Sciences Mirande, Universite de Dijon, BP 138, 21004, Dijon Cedex aut Delachambre Marie Laboratoire de Physiologie Animale et de la Nutrition, Faculte des Sciences Mirande, Universite de Dijon, BP 138, 21004, Dijon Cedex aut Lecerf Jean Laboratoire de Physiologie Animale et de la Nutrition, Faculte des Sciences Mirande, Universite de Dijon, BP 138, 21004, Dijon Cedex aut Lipids Springer-Verlag 25/10(1990-10-01), 653-660 0024-4201 25:10<653 1990 25 11745 https://doi.org/10.1007/BF02536017 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02536017 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bentejac Marc Laboratoire de Physiologie Animale et de la Nutrition, Faculte des Sciences Mirande, Universite de Dijon, BP 138, 21004, Dijon Cedex aut NATIONALLICENCE 700 1- Bugaut Maurice Laboratoire de Physiologie Animale et de la Nutrition, Faculte des Sciences Mirande, Universite de Dijon, BP 138, 21004, Dijon Cedex aut NATIONALLICENCE 700 1- Delachambre Marie Laboratoire de Physiologie Animale et de la Nutrition, Faculte des Sciences Mirande, Universite de Dijon, BP 138, 21004, Dijon Cedex aut NATIONALLICENCE 700 1- Lecerf Jean Laboratoire de Physiologie Animale et de la Nutrition, Faculte des Sciences Mirande, Universite de Dijon, BP 138, 21004, Dijon Cedex aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/10(1990-10-01), 653-660 0024-4201 25:10<653 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer