caa a22 4500 46576617X CHVBK 20180323111914.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF02536012 doi (NATIONALLICENCE)springer-10.1007/BF02536012 Green Floyd Department of Medicine, State University of NY at Buffalo, 3495 Bailey Avenue, 14215, Buffalo, NY aut Transformations of 5-HETE by activated keratinocyte 15-lipoxygenase and the activation mechanism [Elektronische Daten] [Floyd Green] There is convincing evidence that normal cultured human keratinocytes possess a 15-lipoxygenase activity which, however, does not appear to manifest itself without cell membrane damage. When "activated”, this enzyme transforms arachidonic acid into 15-hydroxyeicosatetraenoic acid (15-HETE), and linoleic acid into 13-hydroxyoctadecadienoic acid, presumably by peroxidase action on their respective hydroperoxy intermediates. Normal but notmembrane-damaged keratinocytes metabolize exogenous 5-HETE, principally by esterifying the eicosanoid intact, primarily in the triacylglycerol fraction. In the present study, membrane-damaged keratinocytes were found to transform 5-HETE to 5,15-diHETE and also to a lipoxin-like group of tetraenes. Similar, if not identical, tetraenes were produced by action of the keratinocyte enzyme on 5(S),15(S)-diHETE, which points to the role of the latter as an intermediate between 5-HETE and the tetraenes. A direction for further study of the mechanism of the "activation” step is presented. American Oil Chemists' Society, 1990 Lipids Springer-Verlag 25/10(1990-10-01), 618-623 0024-4201 25:10<618 1990 25 11745 https://doi.org/10.1007/BF02536012 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02536012 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Green Floyd Department of Medicine, State University of NY at Buffalo, 3495 Bailey Avenue, 14215, Buffalo, NY aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/10(1990-10-01), 618-623 0024-4201 25:10<618 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer