caa a22 4500 465766188 CHVBK 20180323111914.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF02536016 doi (NATIONALLICENCE)springer-10.1007/BF02536016 Selective reduction of fatty acid oxidation in colonocytes: Correlation with ulcerative colitis [Elektronische Daten] [W. Roediger, S. Nance] Attempts were made to define which fatty acid (2∶0 to 18∶1) was optimally oxidized by isolated colonocytes (colonic epithelial cells) and to select inhibitors of fatty acid oxidation which would be analogous in their action to the inhibition of fatty acid oxidation observed in colonocytes involved with ulcerative colitis. Isolated colonic epithelial cells of Sprague-Dawley rats were used with 2-mercaptoacetate, dichloroacetate, 3-mercaptopropionate, 4-mercaptobutyrate, 4-sulfatebutyrate, 2-bromobutyrate, sulfite ions and nitrite ions. n-Butyrate (4∶0) was maximmaly oxidized to CO2 and ketone bodies (mean value 5.46 μmol/min/g dry wt). Oxidation of butyrate to CO2 was diminished by 2-bromobutyrate, sulfite ions and all mercapto fatty acids. Both fatty acid oxidation and glucose oxidation were significantly inhibited by 2-bromobutyrate, while mercapto fatty acids and sulfite inhibited fatty acid oxidation (p<0.01) without significantly changing glucose oxidation. Observation with 2-mercaptoacetate and sulfite correlate with early changes of fatty acid oxidation observed in cases of ulcerative colitis, and warrant further study with isolated colonocytes of man. American Oil Chemists' Society, 1990 Roediger W. Cell Physiology Laboratory of the Department of Surgery, Queen Elizabeth Hospital and University of Adelaide, S.A., South Australia, Australia aut Nance S. Cell Physiology Laboratory of the Department of Surgery, Queen Elizabeth Hospital and University of Adelaide, S.A., South Australia, Australia aut Lipids Springer-Verlag 25/10(1990-10-01), 646-652 0024-4201 25:10<646 1990 25 11745 https://doi.org/10.1007/BF02536016 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02536016 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Roediger W. Cell Physiology Laboratory of the Department of Surgery, Queen Elizabeth Hospital and University of Adelaide, S.A., South Australia, Australia aut NATIONALLICENCE 700 1- Nance S. Cell Physiology Laboratory of the Department of Surgery, Queen Elizabeth Hospital and University of Adelaide, S.A., South Australia, Australia aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/10(1990-10-01), 646-652 0024-4201 25:10<646 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer