caa a22 4500 465766196 CHVBK 20180323111914.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF02536011 doi (NATIONALLICENCE)springer-10.1007/BF02536011 Composition of mouse peritoneal macrophage phospholipid molecular species [Elektronische Daten] [Casimir Akoh, Robert Chapkin] The individual molecular species composition of diacyl, alkylacyl and alkenylacyl glycerophospholipids was determined in mouse peritoneal macrophages. A marked deterogeneity in the relative composition (mol%) of macrophage ether and ester phospholipid individual species was noted. High concentrations of 16∶0-20∶4 were found in ether phospholipids such as alkenylacyl glycerophosphoethanolamine (GPE; 27.5 mol%) and alkylacyl glycerophosphocholine (GPC; 16.6%) as compared to mol % levels of 16∶0-20∶4 in diacyl GPE (5.7%) and diacyl GPC (8.1%), respectively. Interestingly, alkenylacyl GPE was highly enriched in 1-ether (16∶0) relative to alkylacyl GPC. The predominant diacyl molecular species in glycerophosphoinositol (GPI) and glycerophosphoserine (GPS) were 18∶0-20∶4 (59.1%) and 16∶0-18∶1 (41.1%), respectively. It is noteworthy that the level of 18∶0-20∶4 was several times higher in diacyl GPI (59.1%) than in diacyl GPS (11.1%), diacyl GPE (25.7%), and diacyl GPC (3.7%). The most abundant molecular species in diacyl GPC and diacyl GPE were 16∶0-18∶1 (29.9%) and 18∶0-20∶4 (25.7%), respectively. The abundance of 20∶4 in ether phospholipids, specifically 16∶0-20∶4 and 18∶0-20∶4, in alkylacyl GPC is significant in view of the role these antecedents play in the biosynthesis of platelet-activating factor (PAF) and 20∶4-derived eicosanoids in stimulated macrophages. The unique molecular species composition of the peritoneal macrophage distinguishes this cell type from others. American Oil Chemists' Society, 1990 Akoh Casimir Molecular and Cell Biology Section, Department of Animal Science, Texas A&M University Station, 77843-2471, College Station, Texas aut Chapkin Robert Molecular and Cell Biology Section, Department of Animal Science, Texas A&M University Station, 77843-2471, College Station, Texas aut Lipids Springer-Verlag 25/10(1990-10-01), 613-617 0024-4201 25:10<613 1990 25 11745 https://doi.org/10.1007/BF02536011 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02536011 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Akoh Casimir Molecular and Cell Biology Section, Department of Animal Science, Texas A&M University Station, 77843-2471, College Station, Texas aut NATIONALLICENCE 700 1- Chapkin Robert Molecular and Cell Biology Section, Department of Animal Science, Texas A&M University Station, 77843-2471, College Station, Texas aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/10(1990-10-01), 613-617 0024-4201 25:10<613 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer