caa a22 4500 465766218 CHVBK 20180323111914.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF02536019 doi (NATIONALLICENCE)springer-10.1007/BF02536019 Carbohydrate content of apolipoprotein B-48 from rat chylomicrons of varying density [Elektronische Daten] [Wieslaw Bochenek, Peter Weber, Regina Slowinska, Gexin Tang, John Rodgers] Monosaccharide composition was determined in apolipoprotein B-48 (apoB) of chylomicrons of rat mesenteric lymph. Chylomicrons were separated into three fractions based on density. Triglyceride and apolipoprotein content were determined in each. ApoB was isolated and quantified using precipitation with isopropanol. Chylomicrons were collected in lymph under normal conditions, and with Poloxalene 2930 when chylomicron secretion was inhibited. Most of the triglyceride was carried in the least dense fraction, while the highest apoB content was in the most dense fraction under normal conditions. Mannose and galactosamine contents of apoB were similar in all fractions while contents of both glucosamine and galactose were highest in the least dense fraction. When chylomicron secretion was inhibited by Poloxalene, the amount of triglyceride recovered in the least dense fraction was significantly reduced. Despite the inhibition of lipid transport in the least dense fraction of chylomicrons by Poloxalene, there was little change in apoB recoveries and in the relative content of various monosaccharides in the apoB from each of the three fractions as compared to results obtained during lipid absorption under normal conditions. In conclusion, carbohydrate composition of apoB of chylomicrons is heterogeneous and varies with chylomicron density. American Oil Chemists' Society, 1990 Bochenek Wieslaw Department of Medicine, Albany Medical College, 122O8, Albany, New York aut Weber Peter Department of Biochemistry, Albany Medical College, 122O8, Albany, New York aut Slowinska Regina Department of Medicine, Albany Medical College, 122O8, Albany, New York aut Tang Gexin Department of Medicine, Albany Medical College, 122O8, Albany, New York aut Rodgers John Department of Medicine, Albany Medical College, 122O8, Albany, New York aut Lipids Springer-Verlag 25/10(1990-10-01), 665-668 0024-4201 25:10<665 1990 25 11745 https://doi.org/10.1007/BF02536019 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02536019 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bochenek Wieslaw Department of Medicine, Albany Medical College, 122O8, Albany, New York aut NATIONALLICENCE 700 1- Weber Peter Department of Biochemistry, Albany Medical College, 122O8, Albany, New York aut NATIONALLICENCE 700 1- Slowinska Regina Department of Medicine, Albany Medical College, 122O8, Albany, New York aut NATIONALLICENCE 700 1- Tang Gexin Department of Medicine, Albany Medical College, 122O8, Albany, New York aut NATIONALLICENCE 700 1- Rodgers John Department of Medicine, Albany Medical College, 122O8, Albany, New York aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/10(1990-10-01), 665-668 0024-4201 25:10<665 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer