caa a22 4500 465766234 CHVBK 20180323111914.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF02536014 doi (NATIONALLICENCE)springer-10.1007/BF02536014 Effects of free fatty acids on the binding of steroid hormones to bovine serum albumin [Elektronische Daten] [Sadao Watanabe, Takayuki Tani, Shigenobu Watanabe, Manabu Senō] The effects of binding of free fatty acids (FFA) to bovine serum albumin (BSA) on steroid hormone binding to BSA were examined. The FFA studied included myristic, palmitic, stearic, oleic and linoleic acids. The binding coefficient K was estimated from the changes in the equilibrium partition coefficient between the aqueous and the hexane phase caused by the addition to BSA to the aqueous phase. A noticeable effect of FFA binding (molar ratio FFA/BSA, 2∶1) on the affinities of α-estradiol, ethynylestradiol and dehydroisoandrosterone to BSA was not observed: however, the affinities of progesterone, androsterone and testosterone were distinctly enhanced by FFA binding. Furthermore, the elution profiles of gel filtration chromatography clearly showed that progesterone and testosterone are easily liberated from the hormone/BSA complexes and that stronger binding of these hormones to BSA is caused by binding of FFA to BSA. The affinity of ethynylestradiol to BSA is stronger than that of progesterone and testosterone and is not affected by palmitic acid binding to BSA. American Oil Chemists' Society, 1990 Watanabe Sadao Kanagawa Prefectural Public Health Laboratory, 52-2 Nakao-cho, Asahi-ku, 241, Yokohama aut Tani Takayuki Kanagawa Prefectural Public Health Laboratory, 52-2 Nakao-cho, Asahi-ku, 241, Yokohama aut Watanabe Shigenobu Kanagawa Prefectural Public Health Laboratory, 52-2 Nakao-cho, Asahi-ku, 241, Yokohama aut Senō Manabu Institute of Industrial Science, University of Tokyo, 7-22-1, Roppongi Minato-ku, 106, Tokyo, Japan aut Lipids Springer-Verlag 25/10(1990-10-01), 633-638 0024-4201 25:10<633 1990 25 11745 https://doi.org/10.1007/BF02536014 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02536014 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Watanabe Sadao Kanagawa Prefectural Public Health Laboratory, 52-2 Nakao-cho, Asahi-ku, 241, Yokohama aut NATIONALLICENCE 700 1- Tani Takayuki Kanagawa Prefectural Public Health Laboratory, 52-2 Nakao-cho, Asahi-ku, 241, Yokohama aut NATIONALLICENCE 700 1- Watanabe Shigenobu Kanagawa Prefectural Public Health Laboratory, 52-2 Nakao-cho, Asahi-ku, 241, Yokohama aut NATIONALLICENCE 700 1- Senō Manabu Institute of Industrial Science, University of Tokyo, 7-22-1, Roppongi Minato-ku, 106, Tokyo, Japan aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/10(1990-10-01), 633-638 0024-4201 25:10<633 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer