caa a22 4500 465766382 CHVBK 20180323111915.0 cr unu---uuuuu 170327e19900401xx s 000 0 eng 10.1007/BF02535752 doi (NATIONALLICENCE)springer-10.1007/BF02535752 Measurement of malondialdehyde by high performance liquid chromatography with fluorescence detection [Elektronische Daten] [Vickie Tatum, Charus Changchit, Ching Chow] A method has been developed to measure malondial-dehyde (MDA) in biological systems. MDA was reacted with 2-thiobarbituric acid (TBA) in the presence of butylated hydroxytoluene (BHT) to minimize formation of artifacts. Initial separation of the TBA-MDA adduct was accomplished by isobutanol extraction. Further elimination and separation of interfering substances was achieved by high performance liquid chromatography. The mobile phase consisted of a 1∶1 (v/v) mixture of methanol and water with 0.05% (w/v) tetrabutyl ammonium dihydrogen phosphate added as an ion pairing reagent. At a flow rate of 1 ml/min, the TBA-MDA adduct was eluted from a 15-cm, c-18, reversed phase column in approximately 4.9 min. The TBA-MDA adduct was quantitated with a fluorescence detector set at 515 nm excitation and 550 nm emission. Using this method, picomole quantities of MDA can be easily detected in plasma and liver samples. American Oil Chemists' Society, 1990 Tatum Vickie Department of Nutrition and Food Science and Graduate Center for Toxicology, University of Kentucky, 40506-0054, Lexington, KY aut Changchit Charus Department of Nutrition and Food Science and Graduate Center for Toxicology, University of Kentucky, 40506-0054, Lexington, KY aut Chow Ching Department of Nutrition and Food Science and Graduate Center for Toxicology, University of Kentucky, 40506-0054, Lexington, KY aut Lipids Springer-Verlag 25/4(1990-04-01), 226-229 0024-4201 25:4<226 1990 25 11745 https://doi.org/10.1007/BF02535752 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02535752 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tatum Vickie Department of Nutrition and Food Science and Graduate Center for Toxicology, University of Kentucky, 40506-0054, Lexington, KY aut NATIONALLICENCE 700 1- Changchit Charus Department of Nutrition and Food Science and Graduate Center for Toxicology, University of Kentucky, 40506-0054, Lexington, KY aut NATIONALLICENCE 700 1- Chow Ching Department of Nutrition and Food Science and Graduate Center for Toxicology, University of Kentucky, 40506-0054, Lexington, KY aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/4(1990-04-01), 226-229 0024-4201 25:4<226 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer