caa a22 4500 465766404 CHVBK 20180323111915.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF02538085 doi (NATIONALLICENCE)springer-10.1007/BF02538085 Effect of temperature on the incorporation into phospholipid classes and metabolism via desaturation and elongation of n−3 and n−6 polyunsaturated fatty acids in fish cells in culture [Elektronische Daten] [Douglas Tocher, John Sargent] The incorporation of 18∶2n−6, 18∶3n−3, 20∶4n−6 and 20∶5n−3 was greater at 10°C than at 22°C in Atlantic salmon (AS), rainbow trout (RTG-2) and turbot (TF) cells. However, there were generally no significant differences between the amount of incorporation of all four polyunsaturated fatty acids (PUFA) into total lipid within a cell type at either 22°C or 10°C. The distributions of the PUFA between individual phospholipid classes at 22°C was essentially the same in AS and TF cells—with the C18 PUFA the order of incorporation in these cells was phosphatidylcholine (PC) > phosphatidylethanolamine (PE) > phosphatidic acid/cardiolipin (PA/CL); with 20∶4n−6 the order was PE and phosphatidylinositol (PI)>PC; with 20∶5n−3, PE>PC. In RTG-2 cells at 22°C the distributions of the C18 PUFA were similar to the other cell lines, but with 20∶4n−6 the order was PC>PI>PE, and with 20∶5n−3 it was PC>PE. At 10°C the incorporation of C18 PUFA into PC increased and into PE and PA/CL decreased, in general, in all cell lines. Incorporation of 20∶5n−3 into PC and PE was increased and decreased at 10°C, respectively, in AS and TF cells, whereas in RTG-2 cells the changes at 10°C were opposite i.e., increased in PE and decreased in PC. With 20∶4n−6, incorporation into PC at 10°C was increased in all cell lines with decreased incorporation into PI in AS and RTG-2 cells and into PE in AS and TF cells, whereas incorporation of 20∶4n−6 into PE increased in RTG-2 cells. The metabolismvia desaturation and elongation of the n−3 PUFA was greater than that of the equivalent n−6 PUFA in all cell lines, irrespective of temperature. There was less conversion of the C18 PUFA at 10°C than at 22°C in RTG-2 and TF cells, but the conversion of 18∶3n−3 by AS cells was increased at 10°C. Temperature had no effect on the conversion of the C20 PUFA. American Oil Chemists' Society (AOCS), 1990 Tocher Douglas NERC Unit of Aquatic Biochemistry, School of Natural Sciences, University of Stirling, FK9 4LA, Stirling, Scotland aut Sargent John NERC Unit of Aquatic Biochemistry, School of Natural Sciences, University of Stirling, FK9 4LA, Stirling, Scotland aut Lipids Springer-Verlag 25/8(1990-08-01), 435-442 0024-4201 25:8<435 1990 25 11745 https://doi.org/10.1007/BF02538085 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02538085 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tocher Douglas NERC Unit of Aquatic Biochemistry, School of Natural Sciences, University of Stirling, FK9 4LA, Stirling, Scotland aut NATIONALLICENCE 700 1- Sargent John NERC Unit of Aquatic Biochemistry, School of Natural Sciences, University of Stirling, FK9 4LA, Stirling, Scotland aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/8(1990-08-01), 435-442 0024-4201 25:8<435 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer