caa a22 4500 465766412 CHVBK 20180323111915.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF02538093 doi (NATIONALLICENCE)springer-10.1007/BF02538093 The response to endotoxin in guinea pigs after intravenous black currant seed oil [Elektronische Daten] [Yulia Hirschberg, Alan Shackelford, Edward Mascioli, Vigen Babayan, Bruce Bistrian, George Blackburn] The influence on the metabolic response to endotoxin of three days of total parenteral nutrition with lipids high in gammalinolenic acid (18∶3ω6, GLA) compared to soy oil (SO) was examined in acute operatively stressed guinea pigs. GLA is the precursor of dihomogammalinolenic acid (DHLA), the substrate for synthesis of "1” series prostaglandins such as PGE1, which have previously been shown to be protective in endotoxin lung injury and traumatic shock. Guinea pigs fed an intravenous diet containing black currant seed oil (BCO) emulsion (20% GLA) or soy oil emulsion (0% GLA) for 2.5 days had their arterial pH, pCO2, pO2, and bicarbonate measured at baseline and hourly during a 7-hr infusion of endotoxin (lipopolysaccharide (LPS), 2mg/kg) or saline. Plasma lactate and fatty acid profile analyses were performed at the end of the LPS infusion. Increased levels of GLA and DHLA were present in the plasma phospholipid fraction of animals fed the black currant seed oil diet, while soyfed animals had only trace amounts of GLA. In addition, the ratio of DHLA to arachidonate was higher in animals receiving the black currant seed oil total parenteral nutrition (TPN). After 2 hr of LPS infusion, all animals exhibited the typical shock response resulting in metabolic acidosis characterized by a significant (p<0.05) drop in pH from 7.34±.02 (SO) and 7.39±.02 (BCO) at baseline to 7.14±.05 and 7.22±.04 by 7 hr for SO and BCO groups, respectively. Plasma lactate values at the end of the infusion were significantly elevated compared to saline in both groups (p<0.05). Guinea pigs fed GLA showed no improvement over soy-fed animals, and actually exhibited a more pronounced metabolic acidosis and increased mortality (50% vs 0%) over the 7 hr of LPS infusion. No beneficial effects of a diet high in GLA were observed in the present study. American Oil Chemists' Society (AOCS), 1990 Hirschberg Yulia Laboratory of Nutrition/Infection, New England Deaconess Hospital, 185 Pilgrim Rd., 02215, Boston, MA aut Shackelford Alan Laboratory of Nutrition/Infection, New England Deaconess Hospital, 185 Pilgrim Rd., 02215, Boston, MA aut Mascioli Edward Laboratory of Nutrition/Infection, New England Deaconess Hospital, 185 Pilgrim Rd., 02215, Boston, MA aut Babayan Vigen Laboratory of Nutrition/Infection, New England Deaconess Hospital, 185 Pilgrim Rd., 02215, Boston, MA aut Bistrian Bruce Laboratory of Nutrition/Infection, New England Deaconess Hospital, 185 Pilgrim Rd., 02215, Boston, MA aut Blackburn George Laboratory of Nutrition/Infection, New England Deaconess Hospital, 185 Pilgrim Rd., 02215, Boston, MA aut Lipids Springer-Verlag 25/8(1990-08-01), 491-496 0024-4201 25:8<491 1990 25 11745 https://doi.org/10.1007/BF02538093 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02538093 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hirschberg Yulia Laboratory of Nutrition/Infection, New England Deaconess Hospital, 185 Pilgrim Rd., 02215, Boston, MA aut NATIONALLICENCE 700 1- Shackelford Alan Laboratory of Nutrition/Infection, New England Deaconess Hospital, 185 Pilgrim Rd., 02215, Boston, MA aut NATIONALLICENCE 700 1- Mascioli Edward Laboratory of Nutrition/Infection, New England Deaconess Hospital, 185 Pilgrim Rd., 02215, Boston, MA aut NATIONALLICENCE 700 1- Babayan Vigen Laboratory of Nutrition/Infection, New England Deaconess Hospital, 185 Pilgrim Rd., 02215, Boston, MA aut NATIONALLICENCE 700 1- Bistrian Bruce Laboratory of Nutrition/Infection, New England Deaconess Hospital, 185 Pilgrim Rd., 02215, Boston, MA aut NATIONALLICENCE 700 1- Blackburn George Laboratory of Nutrition/Infection, New England Deaconess Hospital, 185 Pilgrim Rd., 02215, Boston, MA aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/8(1990-08-01), 491-496 0024-4201 25:8<491 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer