caa a22 4500 465766420 CHVBK 20180323111915.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF02538087 doi (NATIONALLICENCE)springer-10.1007/BF02538087 The association of lysophosphatidylcholine with isolated cardiac myocytes [Elektronische Daten] [Ricky Man, Anne Kinnaird, Ivan Bihler, Patrick Choy] The ability of exogenous lysophosphatidylcholine to produce electrophysiological derangements and cardiac arrhythmias in the heart has been documented. The action of lysophosphatidylcholine is thought to be mediated via its association with the membrane. The present study examined the nature of the association of lysophosphatidylcholine with isolated rat myocyte membrane. The association was studied by incubating myocytes in a lysophosphatidylcholine-containing medium. The association of lysophosphatidylcholine with the myocyte sarcolemma was not affected by palmitic acid and glycerophosphocholine but was reduced by platelet-activating factor (PAF). The addition of albumin (5 mg/mL) at the end of the incubation period effectively removed the lysophosphatidylcholine from the myocytes. Our results suggest that most of the lysophosphatidylcholine in isolated myocytes was associated preferentially with the outer leaflet of the myocyte sarcolemma. This type of association might be responsible for the lysophosphatidylcholine-induced electrophysiological alterations in the heart. American Oil Chemists' Society (AOCS), 1990 Man Ricky Department of Pharmacology and Therapeutics, University of Manitoba, R3E 0W3, Winnipeg, Manitoba, Canada aut Kinnaird Anne Department of Pharmacology and Therapeutics, University of Manitoba, R3E 0W3, Winnipeg, Manitoba, Canada aut Bihler Ivan Department of Pharmacology and Therapeutics, University of Manitoba, R3E 0W3, Winnipeg, Manitoba, Canada aut Choy Patrick Department of Biochemistry and Molecular Biology, University of Manitoba, R3E 0W3, Winnipeg, Manitoba, Canada aut Lipids Springer-Verlag 25/8(1990-08-01), 450-454 0024-4201 25:8<450 1990 25 11745 https://doi.org/10.1007/BF02538087 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02538087 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Man Ricky Department of Pharmacology and Therapeutics, University of Manitoba, R3E 0W3, Winnipeg, Manitoba, Canada aut NATIONALLICENCE 700 1- Kinnaird Anne Department of Pharmacology and Therapeutics, University of Manitoba, R3E 0W3, Winnipeg, Manitoba, Canada aut NATIONALLICENCE 700 1- Bihler Ivan Department of Pharmacology and Therapeutics, University of Manitoba, R3E 0W3, Winnipeg, Manitoba, Canada aut NATIONALLICENCE 700 1- Choy Patrick Department of Biochemistry and Molecular Biology, University of Manitoba, R3E 0W3, Winnipeg, Manitoba, Canada aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/8(1990-08-01), 450-454 0024-4201 25:8<450 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer