caa a22 4500 465766455 CHVBK 20180323111915.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF02538089 doi (NATIONALLICENCE)springer-10.1007/BF02538089 Gas chromatographic analysis of reactive carbonyl compounds formed from lipids upon UV-irradiation [Elektronische Daten] [K. Dennis, T. Shibamoto] Peroxidation of lipids produces carbonyl compounds; some of these, e.g., malonaldehyde and 4-hydroxynonenal, are genotoxic because of their reactivity with biological nucleophiles. Analysis of the reactive carbonyl compounds is often difficult. The methylhydrazine method developed for malonaldehyde analysis was applied to simultaneously measure the products formed from linoleic acid, linolenic acid, arachidonic acid, and squalene upon ultraviolet-irradiation (UV-irradiation). The photoreaction products, saturated monocarbonyl, α,β-unsaturated carbonyls, and β-dicarbonyls, were derivatized with methylhydrazine to give hydrazones, pyrazolines, and pyrazoles, respectively. The derivatives were analyzed by gas chromatography and gas chromatography-mass spectrometry. Lipid peroxidation products identified included formaldehyde, acetaldehyde, acrolein, malonaldehyde, n-hexanal, and 4-hydroxy-2-nonenal. Malonaldehyde levels formed upon 4 hr of irradiation were 0.06 μg/mg from squalene, 2.4 μg/mg from linolenic acid, and 5.7 μg/mg from arachidonic acid. Significant levels of acrolein (2.5 μg/mg) and 4-hydroxy-2-nonenal (0.17 μg/mg) were also produced from arachidonic acid upon 4 hr irradiation. American Oil Chemists' Society (AOCS), 1990 Dennis K. Department of Environmental Toxicology, University of California, 95616, Davis, CA aut Shibamoto T. Department of Environmental Toxicology, University of California, 95616, Davis, CA aut Lipids Springer-Verlag 25/8(1990-08-01), 460-464 0024-4201 25:8<460 1990 25 11745 https://doi.org/10.1007/BF02538089 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02538089 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dennis K. Department of Environmental Toxicology, University of California, 95616, Davis, CA aut NATIONALLICENCE 700 1- Shibamoto T. Department of Environmental Toxicology, University of California, 95616, Davis, CA aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/8(1990-08-01), 460-464 0024-4201 25:8<460 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer