caa a22 4500 465766501 CHVBK 20180323111915.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF02538094 doi (NATIONALLICENCE)springer-10.1007/BF02538094 Uptake, incorporation and metabolism of (3H)triolein in the isolated perfused rabbit heart [Elektronische Daten] [Margaret Weis, Anthony Palazzo, J. Williams Jr., Kafait Malik] The purpose of these experiments was to study the uptake and metabolism of exogenous triglyceride in the isolated perfused rabbit heart. When infused into the rabbit heart, [9,10-3H(N)]triolein was retained and incorporated into a lipid fraction that had the chromatographic mobility of authentic triolein. Incorporation of labeled triolein was not likely to be the result of a lipoprotein lipase-mediated lipolysis/resynthesis cycle, since: (i) The distribution of radioactivity following administration of [3H]oleic acid was markedly different from the distribution of radioactivity following the administration of [3H]triolein; (ii) heparin was administered to the rabbits at the time of sacrifice; and (iii) the hearts were perfused with a protein-free buffer for 20 min prior to the labelling period. When isoproterenol was administered to hearts labelled with [3H]triolein, there was an increased output of total radioactivity, composed of labelled free fatty acids, diacylglycerol and monoacylglycerol. In these same hearts, there was an increased output of glycerol in response to isoproterenol. However, following the administration of bradykinin or angiotensin II, neither the radioactivity nor the glycerol content of the perfusate was changed. These data suggest that [3H]triolein is selectively incorporated into the triglyceride pool of the isolated perfused rabbit heart. Furthermore, this [3H]triolein is available to hormonally-activated lipolytic enzymes. American Oil Chemists' Society (AOCS), 1990 Weis Margaret Department of Pharmacology, School of Medicine, University of Tennessee Center for the Health Science, Woodland Avenue and 43rd St., 38163, Memphis, Tennessee aut Palazzo Anthony Department of Pharmacology, School of Medicine, University of Tennessee Center for the Health Science, Woodland Avenue and 43rd St., 38163, Memphis, Tennessee aut Williams Jr. J. Department of Pharmacology, School of Medicine, University of Tennessee Center for the Health Science, Woodland Avenue and 43rd St., 38163, Memphis, Tennessee aut Malik Kafait Department of Pharmacology, School of Medicine, University of Tennessee Center for the Health Science, Woodland Avenue and 43rd St., 38163, Memphis, Tennessee aut Lipids Springer-Verlag 25/8(1990-08-01), 497-503 0024-4201 25:8<497 1990 25 11745 https://doi.org/10.1007/BF02538094 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02538094 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Weis Margaret Department of Pharmacology, School of Medicine, University of Tennessee Center for the Health Science, Woodland Avenue and 43rd St., 38163, Memphis, Tennessee aut NATIONALLICENCE 700 1- Palazzo Anthony Department of Pharmacology, School of Medicine, University of Tennessee Center for the Health Science, Woodland Avenue and 43rd St., 38163, Memphis, Tennessee aut NATIONALLICENCE 700 1- Williams Jr J. Department of Pharmacology, School of Medicine, University of Tennessee Center for the Health Science, Woodland Avenue and 43rd St., 38163, Memphis, Tennessee aut NATIONALLICENCE 700 1- Malik Kafait Department of Pharmacology, School of Medicine, University of Tennessee Center for the Health Science, Woodland Avenue and 43rd St., 38163, Memphis, Tennessee aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/8(1990-08-01), 497-503 0024-4201 25:8<497 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer