caa a22 4500 465766528 CHVBK 20180323111915.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF02544384 doi (NATIONALLICENCE)springer-10.1007/BF02544384 Incorporation of hormone-sensitive lipase into phosphatidylcholine vesicles [Elektronische Daten] [Cecilia Holm, Gudrun Fredrikson, Roger Sundler, Per Belfrage] Enzymatically active, detergent-solubilized purified hormone-sensitive lipase (HSL) was incorporated into phosphatidylcholine (PC) vesicles, using a detergent-dialysis procedure with small PC vesicles, obtained by sonication, as phospholipid source and CHAPS, a zwitterionic bile-salt derivative, as detergent. Association of enzyme protein with the PC vesicles was verified by floatation in a discontinuous dextran gradient and by gel chromatography. An average of 35% of added HSL was incorporated into the vesicles. The vesicles were shown, by quasi-elastic light scattering and electron microscopy, to have a diameter of approximately 160 nm. The vesicleassociated HSL could be phosphorylated by cyclic AMP-dependent protein kinase. The vesicles were stable, both with regard to enzyme activity and size, for at least 4 days when stored at 4°C. The preparation of detergent-free, vesicle-associated and stable HSL provides new possibilities to study some of its properties, and supports and extends the previous report (Holm, C., Fredrikson, G., and Belfrage, P.,J. Biol. Chem. 261, 15659-15661, 1986) which demonstrated the amphiphilic character of HSL. American Oil Chemists' Society, 1990 Holm Cecilia Department of Medical and Physiological Chemistry, University of Lund, S-221 OO, Lund, Sweden aut Fredrikson Gudrun Department of Medical and Physiological Chemistry, University of Lund, S-221 OO, Lund, Sweden aut Sundler Roger Department of Medical and Physiological Chemistry, University of Lund, S-221 OO, Lund, Sweden aut Belfrage Per Department of Medical and Physiological Chemistry, University of Lund, S-221 OO, Lund, Sweden aut Lipids Springer-Verlag 25/5(1990-05-01), 254-259 0024-4201 25:5<254 1990 25 11745 https://doi.org/10.1007/BF02544384 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02544384 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Holm Cecilia Department of Medical and Physiological Chemistry, University of Lund, S-221 OO, Lund, Sweden aut NATIONALLICENCE 700 1- Fredrikson Gudrun Department of Medical and Physiological Chemistry, University of Lund, S-221 OO, Lund, Sweden aut NATIONALLICENCE 700 1- Sundler Roger Department of Medical and Physiological Chemistry, University of Lund, S-221 OO, Lund, Sweden aut NATIONALLICENCE 700 1- Belfrage Per Department of Medical and Physiological Chemistry, University of Lund, S-221 OO, Lund, Sweden aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/5(1990-05-01), 254-259 0024-4201 25:5<254 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer