caa a22 4500 465766595 CHVBK 20180323111915.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF02544388 doi (NATIONALLICENCE)springer-10.1007/BF02544388 Effect of dietary fat on phospholipid class distribution and fatty acid composition in rat fat cell plasma membrane [Elektronische Daten] [K. Khuu Thi-Dinh, Y. Demarne, C. Nicolas, C. Lhuillery] The effect of dietary fats on phospholipid class distribution and fatty acid composition was studied in rat fat cell plasma membrane. Three groups of male Wistar weanling rats were fed for 8 wk three diets differing in the amount and nature of the fats: 1.5% sunflower oil (low fat control; LFC), 10% sunflower oil (high fat, unsaturated; HFU), 1.5% sunflower oil+8.5% cocoa butter (high fat, saturated; HFS). Plasma membranes were prepared from epididymal adipocytes. The amount and type of dietary fat significantly altered membrane phospholipid distribution. Phospholipid content was lowered with HFU as compared to LFC or HFS diets, but no changes were observed for cholesterol. Phosphatidylinositol (PI) and phosphatidylserine (PS) were less affected by dietary changes than were other phospholipid classes. Major changes were detected for phosphatidylcholine (PC), phosphatidylethanolamine (PE) and sphingomyelin (SM) contents. No large changes in PC and PE fatty acid compositions were observed between the LFC and HFS groups, but the HFU diet induced several changes. Correlations with plasma membrane 5′-nucleotidase activities are discussed. American Oil Chemists' Society, 1990 Khuu Thi-Dinh K. Laboratoire de Nutrition et Sécurité, CRJ-INRA, Domaine de Vilvert, 78550, Jouy-en-Josas, France aut Demarne Y. Laboratoire de Nutrition et Sécurité, CRJ-INRA, Domaine de Vilvert, 78550, Jouy-en-Josas, France aut Nicolas C. Laboratoire de Nutrition et Sécurité, CRJ-INRA, Domaine de Vilvert, 78550, Jouy-en-Josas, France aut Lhuillery C. Laboratoire de Nutrition et Sécurité, CRJ-INRA, Domaine de Vilvert, 78550, Jouy-en-Josas, France aut Lipids Springer-Verlag 25/5(1990-05-01), 278-283 0024-4201 25:5<278 1990 25 11745 https://doi.org/10.1007/BF02544388 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02544388 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Khuu Thi-Dinh K. Laboratoire de Nutrition et Sécurité, CRJ-INRA, Domaine de Vilvert, 78550, Jouy-en-Josas, France aut NATIONALLICENCE 700 1- Demarne Y. Laboratoire de Nutrition et Sécurité, CRJ-INRA, Domaine de Vilvert, 78550, Jouy-en-Josas, France aut NATIONALLICENCE 700 1- Nicolas C. Laboratoire de Nutrition et Sécurité, CRJ-INRA, Domaine de Vilvert, 78550, Jouy-en-Josas, France aut NATIONALLICENCE 700 1- Lhuillery C. Laboratoire de Nutrition et Sécurité, CRJ-INRA, Domaine de Vilvert, 78550, Jouy-en-Josas, France aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/5(1990-05-01), 278-283 0024-4201 25:5<278 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer