caa a22 4500 465766609 CHVBK 20180323111915.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF02544382 doi (NATIONALLICENCE)springer-10.1007/BF02544382 The effect of glutathione on the vitamin E requirement for inhibition of liver microsomal lipid peroxidation [Elektronische Daten] [R. Leedle, S. Aust] Vitamin E dependent inhibition of rat liver microsomal lipid peroxidation in an NADPH and ADP-Fe+3 containing system occurred at lower vitamin E concentrations in the presence of glutathione (GSH). Using microsomes from rats fed a vitamin E deficient diet, vitamin E was shown to be rquired for inhibition. Inhibition also required the presence of a storage labile microsomal component, since no inhibition was observed when using microsomes that had been stored for one moth. This observation provides evidence that direct reduction of reversibly oxidized vitamin E by GSH does not appear to contribute significantly to inhibition of peroxidation. During GSH and vitamin E dependent inhibition of lipid peroxidation, vitamin E (reduced form) concentrations remained constant, indicating that GSH maintained vitamin E concentrations. Without GSH, vitamin E concentrations dropped rapidly. By adding vitamin E to microsomes, it was found that inhibition of lipid peroxidation in the presence of GSH occurred at about five-fold less vitamin E than in the absence of GSH. Inhibition at these lower levels of vitamin E was 85-90% complete. Results indicate that GSH can be used to maintain vitamin E (reduced form) concentrations, thereby lowering the concentration of vitamin E necessary to inhibit microsomal lipid peroxidation. American Oil Chemists' Society, 1990 Leedle R. Department of Pathology and Biochemistry, Michigan State University, East Lansing, Michigan aut Aust S. Department of Pathology and Biochemistry, Michigan State University, East Lansing, Michigan aut Lipids Springer-Verlag 25/5(1990-05-01), 241-245 0024-4201 25:5<241 1990 25 11745 https://doi.org/10.1007/BF02544382 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02544382 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Leedle R. Department of Pathology and Biochemistry, Michigan State University, East Lansing, Michigan aut NATIONALLICENCE 700 1- Aust S. Department of Pathology and Biochemistry, Michigan State University, East Lansing, Michigan aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/5(1990-05-01), 241-245 0024-4201 25:5<241 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer