caa a22 4500 465766633 CHVBK 20180323111916.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF02562217 doi (NATIONALLICENCE)springer-10.1007/BF02562217 Solubilization and partial purification of cholinephosphotransferase in hamster tissues [Elektronische Daten] [Kar-min O, Patrick Choy] CDP choline:1,2-diacylglycerol cholinephosphotransferase (EC 2.7.8.2) is located on the cytoplasmic side of the endoplasmic reticulum, and catalyzes the final step in the synthesis of phosphatidylcholine via the CDP choline pathway. The enzyme was solubilized from hamster liver microsomes by 3% Triton QS-15, and partially purified by DEAE-Sepharose chromatography and Sepharose 6B chromatography. The microsomal and partially purified enzymes displayed similar pH profile, and both showed absolute requirement for Mg++ or other divalent cations. The Km values of CDP choline were similar between microsomal and partially purified enzyme, whereas the Km value for diacylglycerol was substantially lowered when the enzyme was partially purified. Hamster heart cholinephosphotransferase was not solubilized by Triton QS-15. American Oil Chemists' Society, 1990 O Kar-min Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Manitoba, 770 Bannatyne Avenue, R3E OW3, Winnipeg, Manitoba, Canada aut Choy Patrick Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Manitoba, 770 Bannatyne Avenue, R3E OW3, Winnipeg, Manitoba, Canada aut Lipids Springer-Verlag 25/2(1990-02-01), 122-124 0024-4201 25:2<122 1990 25 11745 https://doi.org/10.1007/BF02562217 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02562217 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- O Kar-min Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Manitoba, 770 Bannatyne Avenue, R3E OW3, Winnipeg, Manitoba, Canada aut NATIONALLICENCE 700 1- Choy Patrick Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Manitoba, 770 Bannatyne Avenue, R3E OW3, Winnipeg, Manitoba, Canada aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/2(1990-02-01), 122-124 0024-4201 25:2<122 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer