caa a22 4500 465766668 CHVBK 20180323111916.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF02562212 doi (NATIONALLICENCE)springer-10.1007/BF02562212 Synergistic activation of CTP:phosphocholine cytidylyltransferase by phosphatidylethanolamine and oleic acid [Elektronische Daten] [Richard Sleight, Hong-Ngoc Thi Dao] CTP:phosphocholine cytidylyltransferase present in rat liver cytosol was activated almost 30-fold when assayed in the presence of liposomes containing 60 mole % dioleoyl phosphatidylethanolamine (DOPE). During the assay, some of the DOPE was degraded to lysoPE and oleic acid. Whereas cytidylyltransferase activity was not affected when assayed in the presence of liposomes containing lysoPE, liposomes containing oleic acid activated the enzyme. Activation by oleic acid could be eliminated by the addition of fatty acid-free bovine serum albumin (BSA) to the assay. When cytidylyltransferase activity was measured in the presence of both BSA and liposomes containing DOPE, enzyme activity was increased almost 20-fold, as compared with assays performed in the absence of added lipid. The 1.5-fold difference in cytidylyltransferase activity observed when cytosol was assayed with DOPE containing liposomes in the absence or presence of BSA (30-fold stimulation vs 20-fold stimulation) cannot be explained by the loss of activation attributable to oleic acid alone. Activation of the enzyme in the presence of liposomes containing DOPE and oleic acid is several-fold greater than the sum of the activations caused by the individual compounds. These data suggest that PE and oleic acid act synergistically in activating the cytidylyltranferase. American Oil Chemists' Society, 1990 Sleight Richard Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, 231 Bethesda Avenue, 45267-O524, Cincinnati, Ohio aut Thi Dao Hong-Ngoc Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, 231 Bethesda Avenue, 45267-O524, Cincinnati, Ohio aut Lipids Springer-Verlag 25/2(1990-02-01), 100-107 0024-4201 25:2<100 1990 25 11745 https://doi.org/10.1007/BF02562212 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02562212 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sleight Richard Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, 231 Bethesda Avenue, 45267-O524, Cincinnati, Ohio aut NATIONALLICENCE 700 1- Thi Dao Hong-Ngoc Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, 231 Bethesda Avenue, 45267-O524, Cincinnati, Ohio aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/2(1990-02-01), 100-107 0024-4201 25:2<100 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer