caa a22 4500 465766676 CHVBK 20180323111916.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF02562210 doi (NATIONALLICENCE)springer-10.1007/BF02562210 Biochemical and functional abnormalities in hypercholesterolemic rabbit platelets [Elektronische Daten] [K. Dalal, S. Ebbe, E. Mazoyer, D. Carpenter, T. Yee] This study was designed to elucidate changes in rabbit platelet lipids induced by a cholesterol rich diet and to explore the possible correlation of these lipid changes with platelet abnormalities. Pronounced biochemical alterations were observed when serum cholesterol levels of 700-1000 mg% were reached. Hypercholesterolemic (HC) platelets contained 37% more neutral lipids and 16% less phospholipids than the controls. Lysolecithin, cholesterol esters and phosphatidylinositol (PI) levels were increased in HC platelets, and the levels of phosphatidylcholine (PC) were decreased. The cholesterol/phospholipid molar ratio of lipidemic platelets increased from 0.55±0.011 to 0.89±0.016 (P<0.01) in eight weeks. HC platelets had 90% more arachidonic acid (AA) in the PI than normal platelets. No significant changes in AA of PC were observed. Platelet function was monitored by the uptake and release of [14C]serotonin in platelet rich plasma (PRP), using varying concentrations of collagen as an aggregating agent. The uptake of [14C]serotonin in HC and normal platelets ranged from 78-94%. The percent of [14C]serotonin released from normal and HC platelets was proportional to the concentration of collagen. However, lipidemic platelets were hyperreactive to low concentrations of collagen. Incorporation of 50 μM acetylsalicylic acid into the aggregating medium suppressed the release of [14C]serotonin in normal PRP by more than 90%, but had only a partial effect on lipidemic PRP. American Oil Chemists' Society, 1990 Dalal K. Lawrence Berkeley Laboratory, 74-157, University of California, 1 Cyclotron Road, 94720, Berkeley, CA aut Ebbe S. Lawrence Berkeley Laboratory, 74-157, University of California, 1 Cyclotron Road, 94720, Berkeley, CA aut Mazoyer E. Lawrence Berkeley Laboratory, 74-157, University of California, 1 Cyclotron Road, 94720, Berkeley, CA aut Carpenter D. Lawrence Berkeley Laboratory, 74-157, University of California, 1 Cyclotron Road, 94720, Berkeley, CA aut Yee T. Lawrence Berkeley Laboratory, 74-157, University of California, 1 Cyclotron Road, 94720, Berkeley, CA aut Lipids Springer-Verlag 25/2(1990-02-01), 86-92 0024-4201 25:2<86 1990 25 11745 https://doi.org/10.1007/BF02562210 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02562210 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dalal K. Lawrence Berkeley Laboratory, 74-157, University of California, 1 Cyclotron Road, 94720, Berkeley, CA aut NATIONALLICENCE 700 1- Ebbe S. Lawrence Berkeley Laboratory, 74-157, University of California, 1 Cyclotron Road, 94720, Berkeley, CA aut NATIONALLICENCE 700 1- Mazoyer E. Lawrence Berkeley Laboratory, 74-157, University of California, 1 Cyclotron Road, 94720, Berkeley, CA aut NATIONALLICENCE 700 1- Carpenter D. Lawrence Berkeley Laboratory, 74-157, University of California, 1 Cyclotron Road, 94720, Berkeley, CA aut NATIONALLICENCE 700 1- Yee T. Lawrence Berkeley Laboratory, 74-157, University of California, 1 Cyclotron Road, 94720, Berkeley, CA aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/2(1990-02-01), 86-92 0024-4201 25:2<86 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer