caa a22 4500 465766722 CHVBK 20180323111916.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF02562214 doi (NATIONALLICENCE)springer-10.1007/BF02562214 Lability of red blood cell membranes to lipid peroxidation: Application to humans fed polyunsaturated lipids [Elektronische Daten] [Cesar Fraga, A. Tappel, Brian Leibovitz, Franz Kuypers, Daniel Chiu, James Iacono, Darshan Kelley] Red blood cell membranes (RBCM) were used to estimate human red blood cell lability to lipid peroxidationin vitro. RBCM were prepared from blood collected from humans fed diets with either 3 or 15% polyunsaturated fatty acids for 80 days. RBCM were isolated by centrifugation, and oxidative stress was induced byin vitro incubation with 0.1 or 0.5 mM tert-butyl hydroperoxide (t-BOOH) in the presence of 0.5 mg added hemoglobin. Lipid Peroxidation was evaluated by measurement of thiobarbituric acid-reactive substances (TBARS). Lipid peroxidation correlated with the protein content of RBCM in both noninduced and t-BOOH-induced lipid peroxidation systems. TBARS production was dependent on the amount of t-BOOH added to the RBCM. The production of TBARS by RBCM incubated with 0.5 mM t-BOOH was correlated with the arachidonic acid content in the red blood cells (RBC) from which RBCM were prepared. The methodology developed was useful for comparative estimations of the lability of RBCM to lipid peroxidation. American Oil Chemists' Society, 1990 Fraga Cesar Department of Food Science and Technology, University of California, 1480 Chemistry Annex, 95616, Davis, CA aut Tappel A. Department of Food Science and Technology, University of California, 1480 Chemistry Annex, 95616, Davis, CA aut Leibovitz Brian Department of Food Science and Technology, University of California, 1480 Chemistry Annex, 95616, Davis, CA aut Kuypers Franz Children's Hospital Medical Center, Oakland, California aut Chiu Daniel Children's Hospital Medical Center, Oakland, California aut Iacono James Western Human Nutrition Research Center, Presidio of San Francisco, California aut Kelley Darshan Western Human Nutrition Research Center, Presidio of San Francisco, California aut Lipids Springer-Verlag 25/2(1990-02-01), 111-114 0024-4201 25:2<111 1990 25 11745 https://doi.org/10.1007/BF02562214 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02562214 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fraga Cesar Department of Food Science and Technology, University of California, 1480 Chemistry Annex, 95616, Davis, CA aut NATIONALLICENCE 700 1- Tappel A. Department of Food Science and Technology, University of California, 1480 Chemistry Annex, 95616, Davis, CA aut NATIONALLICENCE 700 1- Leibovitz Brian Department of Food Science and Technology, University of California, 1480 Chemistry Annex, 95616, Davis, CA aut NATIONALLICENCE 700 1- Kuypers Franz Children's Hospital Medical Center, Oakland, California aut NATIONALLICENCE 700 1- Chiu Daniel Children's Hospital Medical Center, Oakland, California aut NATIONALLICENCE 700 1- Iacono James Western Human Nutrition Research Center, Presidio of San Francisco, California aut NATIONALLICENCE 700 1- Kelley Darshan Western Human Nutrition Research Center, Presidio of San Francisco, California aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/2(1990-02-01), 111-114 0024-4201 25:2<111 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer