caa a22 4500 465766803 CHVBK 20180323111916.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF02544032 doi (NATIONALLICENCE)springer-10.1007/BF02544032 Intermembrane cholesterol transfer: Role of sterol carrier proteins and phosphatidylserine [Elektronische Daten] [Friedhelm Schroeder, Peter Butko, Ivan Hapala, Terrence Scallen] The effect of phosphatidylserine and sterol carrier proteins on cholesterol exchange was determined using an assay not requiring separation of donor and acceptor membrane vesicles. Sterol carrier protein-2 (SCP2, also called nonspecific lipid transfer protein), but not fatty acid binding protein (FABP, also called sterol carrier protein), enhanced the initial rate of sterol exchange between neutral zwitterionic phosphatidylcholine small unilamellar vesicles (SUV) 2.3-fold. Phosphatidylserine at 10 mol% increased the initial rate of spontaneous and of SCP2-mediated (but not FABP-mediated) sterol exchange by 22% and 44-fold, respectively. The SCP2 potentiation of sterol transfer was dependent on SCP2 concentration and on phosphatidylserine concentration. The SCP2-mediated sterol transfer was inhibited by a variety of cations including KCl, divalent metal ions, and neomycin. The data suggest that SCP2 increase in activity for sterol transfer may be partly ascribed to charge on the phospholipid. American Oil Chemists' Society, 1985 Schroeder Friedhelm Division of Pharmacology and Medicinal Chemistry, Department of Pharmacology and Cell Biophysics, University of Cincinnati Medical Center, 45267-0004, Cincinnati, Ohio aut Butko Peter Division of Pharmacology and Medicinal Chemistry, Department of Pharmacology and Cell Biophysics, University of Cincinnati Medical Center, 45267-0004, Cincinnati, Ohio aut Hapala Ivan Division of Pharmacology and Medicinal Chemistry, Department of Pharmacology and Cell Biophysics, University of Cincinnati Medical Center, 45267-0004, Cincinnati, Ohio aut Scallen Terrence Department of Biochemistry, School of Medicine, University of New Mexico, 87131, Albuquerque, New Mexico aut Lipids Springer-Verlag 25/11(1990-11-01), 669-674 0024-4201 25:11<669 1990 25 11745 https://doi.org/10.1007/BF02544032 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02544032 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schroeder Friedhelm Division of Pharmacology and Medicinal Chemistry, Department of Pharmacology and Cell Biophysics, University of Cincinnati Medical Center, 45267-0004, Cincinnati, Ohio aut NATIONALLICENCE 700 1- Butko Peter Division of Pharmacology and Medicinal Chemistry, Department of Pharmacology and Cell Biophysics, University of Cincinnati Medical Center, 45267-0004, Cincinnati, Ohio aut NATIONALLICENCE 700 1- Hapala Ivan Division of Pharmacology and Medicinal Chemistry, Department of Pharmacology and Cell Biophysics, University of Cincinnati Medical Center, 45267-0004, Cincinnati, Ohio aut NATIONALLICENCE 700 1- Scallen Terrence Department of Biochemistry, School of Medicine, University of New Mexico, 87131, Albuquerque, New Mexico aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/11(1990-11-01), 669-674 0024-4201 25:11<669 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer