caa a22 4500 465766838 CHVBK 20180323111916.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF02544047 doi (NATIONALLICENCE)springer-10.1007/BF02544047 Separation and quantification of heart and liver phospholipid classes by high-performance liquid chromatography using a new light-scattering detector [Elektronische Daten] [P. Juanéda, G. Rocquelin, P. Astorg] This work describes a one-step separation of rat tissue phospholipid classes by high-performance liquid chromatography (HPLC) using a silica column and a new light-scattering detector (LSD). Complete separation of phosphatidylcholine, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylserine, sphingomyelin, lysophosphatidylethanolamine, and lysophosphatidylcholine was obtained. Direct quantification was achieved after detector calibration for each phospholipid class. The detector response was shown to be linear within the ranges used. The LSD results agreed well with those obtained by phospholipid phosphorus assay. The present method was applied to rat heart and rat liver phospholipid analysis. American Oil Chemists' Society, 1985 Juanéda P. Institut National de la Recherche Agronomique, Station de Recherches sur la Qualité des Aliments de l'Homme, 21034, Dijon cedex, France aut Rocquelin G. Institut National de la Recherche Agronomique, Station de Recherches sur la Qualité des Aliments de l'Homme, 21034, Dijon cedex, France aut Astorg P. Institut National de la Recherche Agronomique, Station de Recherches sur la Qualité des Aliments de l'Homme, 21034, Dijon cedex, France aut Lipids Springer-Verlag 25/11(1990-11-01), 756-759 0024-4201 25:11<756 1990 25 11745 https://doi.org/10.1007/BF02544047 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02544047 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Juanéda P. Institut National de la Recherche Agronomique, Station de Recherches sur la Qualité des Aliments de l'Homme, 21034, Dijon cedex, France aut NATIONALLICENCE 700 1- Rocquelin G. Institut National de la Recherche Agronomique, Station de Recherches sur la Qualité des Aliments de l'Homme, 21034, Dijon cedex, France aut NATIONALLICENCE 700 1- Astorg P. Institut National de la Recherche Agronomique, Station de Recherches sur la Qualité des Aliments de l'Homme, 21034, Dijon cedex, France aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/11(1990-11-01), 756-759 0024-4201 25:11<756 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer