caa a22 4500 465766854 CHVBK 20180323111916.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF02544045 doi (NATIONALLICENCE)springer-10.1007/BF02544045 A method for the quantitative analysis of molecular species of alkylacylglycerol and diacylglycerol [Elektronische Daten] [Thomas Warne, Mitchell Robinson] We describe a method for the quantitative analysis of molecular species of diacylglycerol and alkylacylglycerol as their diradylglycerobenzoate derivatives. Synthetic internal standards were used to provide quantitative determinations of the low levels of diacylglycerol and alkyl-acylglycerol and their individual molecular species in cultured cells. Diradylglycerols were isolated by thin-layer chromatography (TLC), converted to their benzoate derivatives and separated into subclasses by TLC. The molecular species of each subclass were analyzed by reversed-phase high performance liquid chromatography. Thirty-six species of diglyceride-type molecules were identified in Madin-Darby canine kidney cells. These cells were shown to contain 7.88 nmoles of diacylglycerol and 3.97 nmoles of alkylacylglycerol per μmole of phospholipid. Both subclasses contain predominantly monoenoic and saturated species. This technique should be valuable for studies examining the origin and metabolism of these important intracellular mediators. American Oil Chemists' Society, 1985 Warne Thomas Department of Biochemistry, James H. Quillen College of Medicine, East Tennessee State University, 37614, Johnson City, Tennessee aut Robinson Mitchell Department of Biochemistry, James H. Quillen College of Medicine, East Tennessee State University, 37614, Johnson City, Tennessee aut Lipids Springer-Verlag 25/11(1990-11-01), 748-752 0024-4201 25:11<748 1990 25 11745 https://doi.org/10.1007/BF02544045 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02544045 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Warne Thomas Department of Biochemistry, James H. Quillen College of Medicine, East Tennessee State University, 37614, Johnson City, Tennessee aut NATIONALLICENCE 700 1- Robinson Mitchell Department of Biochemistry, James H. Quillen College of Medicine, East Tennessee State University, 37614, Johnson City, Tennessee aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/11(1990-11-01), 748-752 0024-4201 25:11<748 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer