caa a22 4500 465766919 CHVBK 20180323111916.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF02544044 doi (NATIONALLICENCE)springer-10.1007/BF02544044 Rapid analysis of fatty acids in plasma lipids [Elektronische Daten] [Akira Ohta, Michael Mayo, Nancy Kramer, William Lands] A rapid and convenient procedure for the quantitative determination of the fatty acid composition of plasma lipids is described. Human plasma was applied directly to the preadsorbent zones of thin-layer silica gel plates with added antioxidant, internal standards and carriers. The thin-layer chromatography (TLC) plates were partially developed with methanol followed by chloroform/methanol (1∶1, v/v), and then they were fully developed in hexane/diethyl ether/acetic acid (80∶20∶1, v/v/v) to separate the major classes of lipids. Silica gel from regions containing the separated lipids was scraped into screw-capped tubes and treated with boron trifluoride-methanol prior to gas chromatography. The method of direct application to TLC plates gave yields and compositions of fatty acids very similar to the method of applying extracted plasma lipids. This relatively simple method is suitable for analyzing the fatty acids in plasma lipids from a 50 microliter finger-tip blood samples from an individual, and it may be useful in wide-scale screening of different individuals to estimate the relative amounts of ingested polyunsaturated fatty acids. American Oil Chemists' Society, 1985 Ohta Akira Department of Biological Chemistry, University of Illinois at Chicago, 60680, Chicago, Illinois aut Mayo Michael Department of Biological Chemistry, University of Illinois at Chicago, 60680, Chicago, Illinois aut Kramer Nancy Department of Biological Chemistry, University of Illinois at Chicago, 60680, Chicago, Illinois aut Lands William Department of Biological Chemistry, University of Illinois at Chicago, 60680, Chicago, Illinois aut Lipids Springer-Verlag 25/11(1990-11-01), 742-747 0024-4201 25:11<742 1990 25 11745 https://doi.org/10.1007/BF02544044 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02544044 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ohta Akira Department of Biological Chemistry, University of Illinois at Chicago, 60680, Chicago, Illinois aut NATIONALLICENCE 700 1- Mayo Michael Department of Biological Chemistry, University of Illinois at Chicago, 60680, Chicago, Illinois aut NATIONALLICENCE 700 1- Kramer Nancy Department of Biological Chemistry, University of Illinois at Chicago, 60680, Chicago, Illinois aut NATIONALLICENCE 700 1- Lands William Department of Biological Chemistry, University of Illinois at Chicago, 60680, Chicago, Illinois aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/11(1990-11-01), 742-747 0024-4201 25:11<742 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer