caa a22 4500 465766943 CHVBK 20180323111916.0 cr unu---uuuuu 170327e19900401xx s 000 0 eng 10.1007/BF00294237 doi (NATIONALLICENCE)springer-10.1007/BF00294237 Edema formation and cellular alterations following spinal cord injury in the rat and their modification with p -chlorophenylalanine [Elektronische Daten] [H. Sharma, Y. Olsson] Summary: The possibility that serotonin can modify the early pathological sequences occurring in spinal cord trauma was investigated in a rat model. To that end we took advantage of the possibility of influencing serotonin pharmacologically by treating animals with a serotonin synthesis inhibitor, p-chlorophenylalanine (p-CPA) before the production of the injury and compared the results with injured, untreated controls. A unilateral incision was made into the dorsal horn of the lower thoracic cord (about 2.5 mm deep, 4.5 mm long) and the trauma. The injured region from untreated animals showed macroscopically at that time a pronounced swelling and the water content had increased by 3.5% as compared to intact controls. The segments rostral and caudal to the lesion also exhibited a profound increase in water content. Light microscopy revealed a significant expansion of the spinal cord as compared to controls. The swelling was most pronounced in the gray matter on the injured side. Electron microscopy showed distorted neurons, swollen astrocytes and extracellular edema in the gray matter in and around the primary lesion. There was also a sponginess in the surrounding white matter with disruption of myelin, collapsed axons and widened periaxonal spaces. Pretreatment of the rats with p-CPA significantly reduced the swelling of the injured spinal cord and there was no visible expansion. The ipsilateral edema in the central gray matter was considerable less pronounced as compared to that in untreated animals. The increase in water content was less than 1% in these animals. The neuronal and glial cell changes were also markedly reduced in the drugtreated rats. The disruption of myelin and the vacuolation of the gray matter were much less severe. Our results show that p-CPA can markedly modify the edema and the cellular changes occurring in the traumatic spinal injury and indicate that serotonin is somehow involved in the production of the early, and thus important, pathological events. Springer-Verlag, 1990 Trauma nationallicence Spinal cord injury nationallicence Edema nationallicence Serotonin nationallicence p-CPA nationallicence Sharma H. Laboratory of Neuropathology, Institute of Pathology, University Hospital, S-75185, Uppsala, Sweden aut Olsson Y. Laboratory of Neuropathology, Institute of Pathology, University Hospital, S-75185, Uppsala, Sweden aut Acta Neuropathologica Springer-Verlag 79/6(1990-04-01), 604-610 0001-6322 79:6<604 1990 79 401 https://doi.org/10.1007/BF00294237 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00294237 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sharma H. Laboratory of Neuropathology, Institute of Pathology, University Hospital, S-75185, Uppsala, Sweden aut NATIONALLICENCE 700 1- Olsson Y. Laboratory of Neuropathology, Institute of Pathology, University Hospital, S-75185, Uppsala, Sweden aut NATIONALLICENCE 773 0- Acta Neuropathologica Springer-Verlag 79/6(1990-04-01), 604-610 0001-6322 79:6<604 1990 79 401 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer