caa a22 4500 465766986 CHVBK 20180323111917.0 cr unu---uuuuu 170327e19900401xx s 000 0 eng 10.1007/BF00294238 doi (NATIONALLICENCE)springer-10.1007/BF00294238 Budka H. Neurologisches Institut, Universität Wien, Schwarzspanierstrasse 17, A-1090, Wien, Austria aut Human immunodeficiency virus (HIV) envelope and core proteins in CNS tissues of patients with the acquired immune deficiency syndrome (AIDS) [Elektronische Daten] [H. Budka] Summary: Frequency, cellular tropism and relation to pathology of productive infection with human immunodeficiency virus (HIV) in human central nervous system (CNS) were studied. Serial sections of formolfixed and paraffin-embedded CNS tissues from 70 patients (69 with acquired immune deficiency syndrome, AIDS) were immunolabeled with monoclonal antibodies against HIV antigens (Ags) p17, p24, and gp41. Additional and double (immuno)stains were used to identify cell types and opportunistic infectious agents. HIV Ags were detected in 52 cases; they were restricted to cells with characteristics of microglia or macrophages. Anti-gp41, anti-p24, and anti-p17 labeled 50, 33, and 15 cases, respectively. Immunoreactivity for core proteins predominated in mature macrophages and microglia of fully developed lesions; additional immunoreactivity for gp41 was seen in microglia adjacent to, or unassociated with, histopathological lesions. Multifocal and/or diffuse lesions previously suggested as HIV induced because of characteristic histopathology, consistently contained large numbers of cells with HIV Ags (33 cases), confirming their HIV specificity. Isolated labeled microglia without associated pathology, found in seven brains, presumably represent the earliest stage of productive CNS infection by HIV. Lesions of opportunistic infections contained no (34 cases), few (16 cases), or many (4 cases) cells with HIV Ags. These data do not suggest transactivation of local HIV production by opportunistic agents as a frequent event in vivo. Development of specific HIV histopathology appears correlated with the number of productively infected cells. Springer-Verlag, 1990 Acquired immune deficiency syndrome (AIDS) nationallicence Human immunodeficiency virus (HIV) nationallicence Immunocytochemistry nationallicence Macrophage nationallicence Microglia nationallicence Acta Neuropathologica Springer-Verlag 79/6(1990-04-01), 611-619 0001-6322 79:6<611 1990 79 401 https://doi.org/10.1007/BF00294238 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00294238 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Budka H. Neurologisches Institut, Universität Wien, Schwarzspanierstrasse 17, A-1090, Wien, Austria aut NATIONALLICENCE 773 0- Acta Neuropathologica Springer-Verlag 79/6(1990-04-01), 611-619 0001-6322 79:6<611 1990 79 401 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer