caa a22 4500 465766994 CHVBK 20180323111917.0 cr unu---uuuuu 170327e19900401xx s 000 0 eng 10.1007/BF00294242 doi (NATIONALLICENCE)springer-10.1007/BF00294242 Paired helical filaments are not the major binding sites for wheat germ and Dolichos biflorus agglutinins in the neurofibrillary tangles of Alzheimer's disease [Elektronische Daten] [D. Sparkman, S. Hill, C. White III] Summary: The isolated paired helical filaments (PHF) that occur in the neurofibrillary tangles of Alzheimer's disease were assayed to determine if they contained N-acetyl-glucosamine and N-acetyl-galactosamine residues. The enzyme-linked lectin assay was used to detect their total content in the PHF preparation. The assay employed biotinylated Dolichos biflorus and wheat germ agglutinins and was developed with avidin-horseradish peroxidase. The total PHF preparation was shown to contain very little of these glycosylated control proteins. Colloidal gold-labeled lectins were used to study the PHF by electron microscopy to assess whether the minor amount of lectin binding in the total preparation was directly associated with the PHF. These studies showed no significant association of the colloidal gold-labeled lectins with the isolated filaments. We conclude that the PHF themselves contain few or no N-acetyl-glucosamine or N-acetyl-galactosamine residues. Springer-Verlag, 1990 Alzheimer's disease nationallicence Neurofibrillary tangles nationallicence Paired helical filaments nationallicence Glycoproteins nationallicence Lectin-gold complexes nationallicence Sparkman D. Department of Pathology, Division of Neuropathology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, 75235-9072, Dallas, TX, USA aut Hill S. Department of Pathology, Division of Neuropathology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, 75235-9072, Dallas, TX, USA aut White III C. Department of Pathology, Division of Neuropathology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, 75235-9072, Dallas, TX, USA aut Acta Neuropathologica Springer-Verlag 79/6(1990-04-01), 640-646 0001-6322 79:6<640 1990 79 401 https://doi.org/10.1007/BF00294242 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00294242 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sparkman D. Department of Pathology, Division of Neuropathology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, 75235-9072, Dallas, TX, USA aut NATIONALLICENCE 700 1- Hill S. Department of Pathology, Division of Neuropathology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, 75235-9072, Dallas, TX, USA aut NATIONALLICENCE 700 1- White III C. Department of Pathology, Division of Neuropathology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, 75235-9072, Dallas, TX, USA aut NATIONALLICENCE 773 0- Acta Neuropathologica Springer-Verlag 79/6(1990-04-01), 640-646 0001-6322 79:6<640 1990 79 401 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer