caa a22 4500 465767052 CHVBK 20180323111917.0 cr unu---uuuuu 170327e19900401xx s 000 0 eng 10.1007/BF00294235 doi (NATIONALLICENCE)springer-10.1007/BF00294235 Cytoplasmic argyrophilic inclusions in neurons of pontine nuclei in patients with olivopontocerebellar atrophy: immunohistochemical and ultrastructural studies [Elektronische Daten] [S. Kato, H. Nakamura] Summary: Patients with olivopontocerebellar atrophy (OPCA) were studied, and cytoplasmic inclusions were observed in some of the remaining neurons of the pontine nuclei, nuclei reticularis tegmenti pontis and arcuate nuclei. The cytoplasmic argyrophilic inclusions were demonstrated by silver impregnation techniques such as Bielschowsky and Bodian staining. With hematoxylin and eosin stain, the inclusions were sharply demarcated and appeared pale. The inclusions were not stained by the following routine histological methods: Klüver-Barrera, phosphotungstic acid hematoxylin, Holzer, periodic acid-Schiff, Mallory azan, alcian blue, nile blue, Masson trichrome, Congo red, thioflavine S, oil red O and Sudan black B stains. Immunohistochemistry with anti-ubiquitin antiserum showed that these inclusions were ubiquitinated. However, the inclusions did not react with any of the following antibodies (Abs) or antisera: anti-phosphorylated neurofilament (NF) Ab, anti-nonphosphorylated NF Abs (160 and 200 kDa), anti-paired helical filament antiserum, anti-tau antiserum, anti-tubulin Abs (alpha and beta), anti-microtubule-associated proteins antiserum, anti-glial fibrillary acidic protein antiserum, anti-vimentin Ab, anti-desmin Ab, anti-cytokeratin Abs (low and high molecular weights), anti-actin antiserum, anti-skeletal myosin antiserum and anti-myelin basic protein Ab. Ultrastructurally, the inclusion bodies noted in OPCA were composed primarily of fibrils having a width ranging from about 24 to 40 nm, which were entirely coated with osmiophilic granular material along their whole length. They were occasionally intermingled with a few filaments about 10 nm in width. Electron microscopical examination on silver-impregnated specimens revealed that each granule-coated fibril had a great affinity for silver particles. In elucidating the pathogenesis of OPCA, it was considered to be an important neuropathological finding that some of the remaining pontine neurons affected by OPCA developed characteristic cytoplasmic argyrophilic inclusions. Springer-Verlag, 1990 Olivopontocerbellar atrophy nationallicence Argyrophilic inclusion nationallicence Pontine nucleus nationallicence Ubiquitin nationallicence Ultrastructure nationallicence Kato S. Division of Neuropathology, Institute of Neurological Sciences, Tottori University School of Medicine, 86 Nishi-machi, 683, Yongao, Japan aut Nakamura H. Division of Neuropathology, Institute of Neurological Sciences, Tottori University School of Medicine, 86 Nishi-machi, 683, Yongao, Japan aut Acta Neuropathologica Springer-Verlag 79/6(1990-04-01), 584-594 0001-6322 79:6<584 1990 79 401 https://doi.org/10.1007/BF00294235 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00294235 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kato S. Division of Neuropathology, Institute of Neurological Sciences, Tottori University School of Medicine, 86 Nishi-machi, 683, Yongao, Japan aut NATIONALLICENCE 700 1- Nakamura H. Division of Neuropathology, Institute of Neurological Sciences, Tottori University School of Medicine, 86 Nishi-machi, 683, Yongao, Japan aut NATIONALLICENCE 773 0- Acta Neuropathologica Springer-Verlag 79/6(1990-04-01), 584-594 0001-6322 79:6<584 1990 79 401 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer