caa a22 4500 465767079 CHVBK 20180323111917.0 cr unu---uuuuu 170327e19900401xx s 000 0 eng 10.1007/BF00294241 doi (NATIONALLICENCE)springer-10.1007/BF00294241 Localization and significance of peanut agglutinin-binding sites on ependymoma cells [Elektronische Daten] [J. Kuratsu, N. Sueyoshi, Y. Mihara, Y. Ushio] Summary: It has been suggested that peanut agglutinin (PNA)-binding sites in benign and malignant tissues were quite different. To clarify the difference of PNA-binding sites between benign and malignant ependymoma, PNA-binding sites are investigated on the surface membrane of tissues from benign and malignant ependymomas and cultured ependymoma cells. In four of five malignant ependymoma cases, PNA binding occurred in a diffuse cell membrane fashion or granular intracytoplasmic fashion without neuraminidase treatment. On the other hand, PNA binding was observed without neuraminidase treatment in only two of eight benign ependymoma cases. After neuraminidase treatment, all of our benign and malignant ependymoma cases evidenced PNA binding. PNA binding was clearly evident on the cell membrane of ependymoma cells which were able to express organotypic structures, that is, to form ependymal rosettes after neuraminidase treatment. Normal rat ependymal cells showed PNA binding only after neuraminidase treatment. These findings suggest that the masking of PNA-binding sites of ependymoma cells by sialic acid may be correlated with tumor cell differentiation. Springer-Verlag, 1990 Peanut agglutinin (PNA) nationallicence Human brain tumor nationallicence Ependymoma nationallicence Kuratsu J. Department of Neurosurgery, Kumamoto University Medical School, 1-1-1, Honjo, 860, Kumamoto, Japan aut Sueyoshi N. Department of Neurosurgery, Kumamoto University Medical School, 1-1-1, Honjo, 860, Kumamoto, Japan aut Mihara Y. Department of Neurosurgery, Kumamoto University Medical School, 1-1-1, Honjo, 860, Kumamoto, Japan aut Ushio Y. Department of Neurosurgery, Kumamoto University Medical School, 1-1-1, Honjo, 860, Kumamoto, Japan aut Acta Neuropathologica Springer-Verlag 79/6(1990-04-01), 634-639 0001-6322 79:6<634 1990 79 401 https://doi.org/10.1007/BF00294241 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00294241 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kuratsu J. Department of Neurosurgery, Kumamoto University Medical School, 1-1-1, Honjo, 860, Kumamoto, Japan aut NATIONALLICENCE 700 1- Sueyoshi N. Department of Neurosurgery, Kumamoto University Medical School, 1-1-1, Honjo, 860, Kumamoto, Japan aut NATIONALLICENCE 700 1- Mihara Y. Department of Neurosurgery, Kumamoto University Medical School, 1-1-1, Honjo, 860, Kumamoto, Japan aut NATIONALLICENCE 700 1- Ushio Y. Department of Neurosurgery, Kumamoto University Medical School, 1-1-1, Honjo, 860, Kumamoto, Japan aut NATIONALLICENCE 773 0- Acta Neuropathologica Springer-Verlag 79/6(1990-04-01), 634-639 0001-6322 79:6<634 1990 79 401 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer