caa a22 4500 465767117 CHVBK 20180323111917.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00308723 doi (NATIONALLICENCE)springer-10.1007/BF00308723 Rosenthal fibres: an immunohistochemical, ultrastructural and immunoelectron microscopic study [Elektronische Daten] [A. Dinda, C. Sarkar, S. Roy] Summary: The nature of Rosenthal fibres (RF) was investigated in eight cases each of low-grade astrocytoma and reactive gliosis using immunohistochemical (IH) staining for glial fibrillary acidic protein (GFAP), electron microscopy (EM) and immunoelectron microscopy (IEM) by immunogold labelling technique. By IH under light microscopy (LM), three types of RF were seen, uniformly positive (type I), rim positive (type II) and completely negative (type III). EM showed variation in structural pattern of RF. Some RF contained large amount of glial filaments (GF) intermingled with RF while others with a large amount of electron dense material and less GF. Thus, the presence and amount of GF in RF appear to be responsible for the different types of IH staining under LM. IEM showed that all RF including the ones consisting of entirelh amorphous material possess immunoreactivity for GFAP. It is suggested that RF formation is a two-stage process, staring with excessive accumulation of GF within astrocytic processes followed by their gradual alteration into electron-dense amorphous material under the influence of some unknown metablic or other factors. The quantitative analysis of different types of RF suggests a difference in the rate of formation of RF in neoplastic and reactive conditions. Springer-Verlag, 1990 Rosenthal fibers nationallicence Immunohistochemistry nationallicence Ultrastructure nationallicence Neoplastic and reactive astrocytes nationallicence Dinda A. Department of Pathology, All India Institute of Medical Sciences, 110 029, New Delhi, India aut Sarkar C. Department of Pathology, All India Institute of Medical Sciences, 110 029, New Delhi, India aut Roy S. Department of Pathology, All India Institute of Medical Sciences, 110 029, New Delhi, India aut Acta Neuropathologica Springer-Verlag 79/4(1990-01-01), 456-460 0001-6322 79:4<456 1990 79 401 https://doi.org/10.1007/BF00308723 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00308723 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dinda A. Department of Pathology, All India Institute of Medical Sciences, 110 029, New Delhi, India aut NATIONALLICENCE 700 1- Sarkar C. Department of Pathology, All India Institute of Medical Sciences, 110 029, New Delhi, India aut NATIONALLICENCE 700 1- Roy S. Department of Pathology, All India Institute of Medical Sciences, 110 029, New Delhi, India aut NATIONALLICENCE 773 0- Acta Neuropathologica Springer-Verlag 79/4(1990-01-01), 456-460 0001-6322 79:4<456 1990 79 401 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer