caa a22 4500 465767257 CHVBK 20180323111917.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00308710 doi (NATIONALLICENCE)springer-10.1007/BF00308710 Appearance of tubulovesicular structures in experimental Creutzfeldt-Jakob disease and scrapie preceeds the onset of clinical disease [Elektronische Daten] [P. Liberski, R. Yanagihara, C. Gibbs Jr., D. Gajdusek] Summary: We have consistently observed tubulovesicular structures in brain tissues during the terminal stages of naturally occurring and experimentally induced spongiform encephalopathies, irrespective of the host species and virus strain. In NIH Swiss mice inoculated intracerebrally or intraocularly with the Fujisaki strain of Creutzfeldt-Jakob disease (CJD) virus, tubulovesicular structures, measuring 20-50 nm in diameter, were particularly prominent in dilated, pre-and postsynaptic neuronal processes, occasionally being mixed with synaptic vesicles. These structures appeared 13 weeks following intracerebral inoculation, 5 weeks before the onset of clinical signs, when spongiform changes were also detected. The number and density of tubulovesicular structures increased steadily during the course of clinical disease, and were particularly abundant in mice 47 to 51 weeks after intraocular inoculation. In hamsters infected with the 263 K strain of scrapie virus, these structures were initially detected 3 weeks following intracerebral inoculation and increased dramatically at 10 weeks postinoculation. The appearance of tubulovesicular structures before the onset of overt disease in mice inoculated with CJD virus by either the intracerebral or intraocular route, and before the appearance of other neuropathological changes in hamsters infected with scrapie virus, indicate that they represent either a part or aggregate of the infectious virus or a pathological product of the infection. Springer-Verlag, 1990 Creutzfeldt-Jakob disease nationallicence Electron microscopy nationallicence Scrapie nationallicence Tubulovesicular structures nationallicence Liberski P. Laboratory of Central Nervous System Studies, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bldg. 36, Rm. 5B-21, 20892, Bethesda, MD, USA aut Yanagihara R. Laboratory of Central Nervous System Studies, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bldg. 36, Rm. 5B-21, 20892, Bethesda, MD, USA aut Gibbs Jr. C. Laboratory of Central Nervous System Studies, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bldg. 36, Rm. 5B-21, 20892, Bethesda, MD, USA aut Gajdusek D. Laboratory of Central Nervous System Studies, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bldg. 36, Rm. 5B-21, 20892, Bethesda, MD, USA aut Acta Neuropathologica Springer-Verlag 79/4(1990-01-01), 349-354 0001-6322 79:4<349 1990 79 401 https://doi.org/10.1007/BF00308710 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00308710 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liberski P. Laboratory of Central Nervous System Studies, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bldg. 36, Rm. 5B-21, 20892, Bethesda, MD, USA aut NATIONALLICENCE 700 1- Yanagihara R. Laboratory of Central Nervous System Studies, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bldg. 36, Rm. 5B-21, 20892, Bethesda, MD, USA aut NATIONALLICENCE 700 1- Gibbs Jr C. Laboratory of Central Nervous System Studies, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bldg. 36, Rm. 5B-21, 20892, Bethesda, MD, USA aut NATIONALLICENCE 700 1- Gajdusek D. Laboratory of Central Nervous System Studies, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bldg. 36, Rm. 5B-21, 20892, Bethesda, MD, USA aut NATIONALLICENCE 773 0- Acta Neuropathologica Springer-Verlag 79/4(1990-01-01), 349-354 0001-6322 79:4<349 1990 79 401 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer