caa a22 4500 465767346 CHVBK 20180323111918.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF00296118 doi (NATIONALLICENCE)springer-10.1007/BF00296118 Comparison of three silver stains for demonstrating neurofibrillary tangles and neuritic plaques in brain tissue stored for long periods [Elektronische Daten] [G. Wilcock, S. Matthews, T. Moss] Summary: Three methods were compared to find a reliable method for demonstrating neurofibrillary tangles (NFTs) and neuritic plaques (NPs) in brain tissue stored for long periods in formalin or as paraffin blocks. The short-term fixation of tissue, e.g. up to 6 months in formalin does not usually present a problem using any of the three methods tried, e.g. Gallyas, modified Palmgren, or modified Bielschowsky, but once the time lengthens to 6 years or more the demonstration of NFTs and NPs is not so reliable using the first two methods. The modified Bielschowsky method, however, demonstrates well both NFTs and NPs in material stored in formalin or as paraffin blocks for long periods, e.g. 7 years, and also compares favourably with the other methods on freshly processed material and fixed tissue, stored for shorter periods. We also noted as a consistent trend, irrespective of the staining technique employed, the detection of fewer plaques and tangles in material stored in formalin, as opposed to that stored as blocks embedded in paraffin wax. Springer-Verlag, 1990 Neurofibrillary tangles nationallicence Neuritic plaques nationallicence Alzhemer's disease nationallicence Comparison of silver stains nationallicence Old material nationallicence Wilcock G. Department of Care of the Elderly, Frenchay Hospital, BS16 1LE, Bristol, Great Britain aut Matthews S. Department of Care of the Elderly, Frenchay Hospital, BS16 1LE, Bristol, Great Britain aut Moss T. Department of Neuropathology, Frenchay Hospital, BS16 1LE, Bristol, Great Britain aut Acta Neuropathologica Springer-Verlag 79/5(1990-02-01), 566-568 0001-6322 79:5<566 1990 79 401 https://doi.org/10.1007/BF00296118 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00296118 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wilcock G. Department of Care of the Elderly, Frenchay Hospital, BS16 1LE, Bristol, Great Britain aut NATIONALLICENCE 700 1- Matthews S. Department of Care of the Elderly, Frenchay Hospital, BS16 1LE, Bristol, Great Britain aut NATIONALLICENCE 700 1- Moss T. Department of Neuropathology, Frenchay Hospital, BS16 1LE, Bristol, Great Britain aut NATIONALLICENCE 773 0- Acta Neuropathologica Springer-Verlag 79/5(1990-02-01), 566-568 0001-6322 79:5<566 1990 79 401 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer