caa a22 4500 465767362 CHVBK 20180323111918.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF00296110 doi (NATIONALLICENCE)springer-10.1007/BF00296110 Contrary effect of lactic acid on expression of neuron-specific enolase and glial fibrillary acidic protein in human glioma cells [Elektronische Daten] [I. Takeshita, H. Sawa, T. Nakamura, M. Kuramitsu, K. Kitamura, M. Fukui] Summary: We examined the effect of lactic acid on cultured human glioma cell lines expressing glial fibrillary acidic protein (GFAP), vimentin and neuronspecific enolase (NSE). The growth of the cells was inhibited by the lactic acid in a dose-dependent manner. At 56 mM of lactic acid, the surviving cells of the KNS-42-c2 cell line developed slender processes and increasingly formed bizzar giant cells. In an immunofluorescence study of the lactic acid-resistant cells, the GFAP-positive cells prominently decreased in number, while the NSE-positive cells clearly increased. The vimentin was not affected throughout the experiment. After removing lactic acid from the medium, the GFAP-positive cells gradually increased in number. The method of dot immunoassay was useful for quantifying GFAP in cellular extracts. It indicated that the amount of GFAP decreased in the cells cultured with lactate-containing media and increased to the primary values after removing the lactic acid. These results may suggest that the morphological and immunochemical diversities of glioma cells are secondarily affected by cellular microenvironments such as lactic acid. Springer-Verlag, 1990 Lactic acid nationallicence Glial fibrillary acidic protein (GFAP) nationallicence Neuron-specific enolase (NSE) nationallicence Vimentin nationallicence Astrocyte nationallicence Takeshita I. Department of Neurosurgery, Neurological Institute, Faculty of Medicine, Kyushu University 60, Higashi-ku, 812, Fukuoka, Japan aut Sawa H. Department of Neuropathology, Neurological Institute, Faculty of Medicine, Kyushu University 60, Higashi-ku, 812, Fukuoka, Japan aut Nakamura T. Department of Neurosurgery, Neurological Institute, Faculty of Medicine, Kyushu University 60, Higashi-ku, 812, Fukuoka, Japan aut Kuramitsu M. Department of Neuropathology, Neurological Institute, Faculty of Medicine, Kyushu University 60, Higashi-ku, 812, Fukuoka, Japan aut Kitamura K. Department of Neurosurgery, Neurological Institute, Faculty of Medicine, Kyushu University 60, Higashi-ku, 812, Fukuoka, Japan aut Fukui M. Department of Neurosurgery, Neurological Institute, Faculty of Medicine, Kyushu University 60, Higashi-ku, 812, Fukuoka, Japan aut Acta Neuropathologica Springer-Verlag 79/5(1990-02-01), 506-512 0001-6322 79:5<506 1990 79 401 https://doi.org/10.1007/BF00296110 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00296110 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takeshita I. Department of Neurosurgery, Neurological Institute, Faculty of Medicine, Kyushu University 60, Higashi-ku, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Sawa H. Department of Neuropathology, Neurological Institute, Faculty of Medicine, Kyushu University 60, Higashi-ku, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Nakamura T. Department of Neurosurgery, Neurological Institute, Faculty of Medicine, Kyushu University 60, Higashi-ku, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Kuramitsu M. Department of Neuropathology, Neurological Institute, Faculty of Medicine, Kyushu University 60, Higashi-ku, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Kitamura K. Department of Neurosurgery, Neurological Institute, Faculty of Medicine, Kyushu University 60, Higashi-ku, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Fukui M. Department of Neurosurgery, Neurological Institute, Faculty of Medicine, Kyushu University 60, Higashi-ku, 812, Fukuoka, Japan aut NATIONALLICENCE 773 0- Acta Neuropathologica Springer-Verlag 79/5(1990-02-01), 506-512 0001-6322 79:5<506 1990 79 401 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer