caa a22 4500 465767370 CHVBK 20180323111918.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF00296104 doi (NATIONALLICENCE)springer-10.1007/BF00296104 Prominent white matter lesions develop in Mongolian gerbils treated with 100% normobaric oxygen after global brain ischemia [Elektronische Daten] [H. Mickel, O. Kempski, G. Feuerstein, J. Parisi, H. deF. Webster] Summary: Carotid arteries were occluded bilaterally for 15 min in two groups of Mongolian gerbils. The first group received 100% oxygen during the first 3 h of reperfusion. During that period, room air was given to the second group. After 3 h, both groups received room air. Brains of gerbils that died within 14 days after occlusion were removed, fixed in formalin and embedded in paraffin. Gerbils that survived 15-28 days were perfused with formalin before their brains were removed and embedded in paraffin. Adjacent, serially cut sections were stained with luxol fast blue (LFB)-H&E, cresyl violet, according to the Bodian method, or immunocytochemically with antisera raised against myelin basic protein (MBP) and glial fibrillary acidic protein (GFAP). In brain sections of gerbils receiving 3 h of 100% oxygen, there were circumscribed white matter lesions in the corpus striatum, lateral thalamus, mesencephalon and posterior limb of the internal capsule. Myelin sheaths were swollen, fragmented and were less intensely stained by MBP antiserum. MBP and LFB-stained myelin fragments were present extracellularly and in macrophages. Many axons in these areas appeared undamaged. Previously described ischemic changes were found in gray matter and some areas of white matter in both groups. However, neurons in the deeper laminae of the cerebral cortex appeared to be better preserved in gerbils given oxygen. The results suggest that hyperoxia, if present immediately after transient brain ischemia, may damage myelin more severely than other cellular elements. Springer-Verlag, 1990 Brain ischemia nationallicence Demyelination nationallicence Hyperoxia nationallicence Lipid peroxidation nationallicence Myelin nationallicence Mickel H. Laboratory of Experimental Neuropathology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 20892, Bethesda, MD, USA aut Kempski O. Insutut für Chirurgische Forschung, Klinikum Großhadern, Ludwig-Maximilians-Universität, D-8000, München, Federal Republic of Germany aut Feuerstein G. Department of Neurology, Uniformed Services, University of the Health Sciences, 20814, Bethesda, MD, USA aut Parisi J. Department of Neuropathology, Armed Forces Institute of Pathology, 20306-6000, DC, USA aut deF. Webster H. Laboratory of Experimental Neuropathology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 20892, Bethesda, MD, USA aut Acta Neuropathologica Springer-Verlag 79/5(1990-02-01), 465-472 0001-6322 79:5<465 1990 79 401 https://doi.org/10.1007/BF00296104 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00296104 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mickel H. Laboratory of Experimental Neuropathology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 20892, Bethesda, MD, USA aut NATIONALLICENCE 700 1- Kempski O. Insutut für Chirurgische Forschung, Klinikum Großhadern, Ludwig-Maximilians-Universität, D-8000, München, Federal Republic of Germany aut NATIONALLICENCE 700 1- Feuerstein G. Department of Neurology, Uniformed Services, University of the Health Sciences, 20814, Bethesda, MD, USA aut NATIONALLICENCE 700 1- Parisi J. Department of Neuropathology, Armed Forces Institute of Pathology, 20306-6000, DC, USA aut NATIONALLICENCE 700 1- deF. Webster H. Laboratory of Experimental Neuropathology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 20892, Bethesda, MD, USA aut NATIONALLICENCE 773 0- Acta Neuropathologica Springer-Verlag 79/5(1990-02-01), 465-472 0001-6322 79:5<465 1990 79 401 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer