caa a22 4500 465767397 CHVBK 20180323111918.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF00296112 doi (NATIONALLICENCE)springer-10.1007/BF00296112 Cross-reactivity of monoclonal antibody Leu-19 with some neuroectodermal and other non-immune tissues [Elektronische Daten] [R. Nawrotzki, A. Starzinski-Powitz, F. Weber, R. Schröder] Summary: Leu-19 antigen is a 200-220 kDa surface glycoprotein, initially detected on natural killer (NK) cells exhibiting non-major histocompatibility complex-restricted cytotoxity. Using a monoclonal antibody (mAb) directed against the Leu-19 molecule, we were unable to identify NK cells immunohistologically in cryostat sections of neuroectodermal tumors. Instead, mAb Leu-19 cross-reacted with the surfaces of the tumor cells, except for melanomas. It stained mesodermal tumor cells less intensely and did not stain those of carcinoma metastases. In three plasmocytomas, cells were observed, most likely NK cells, which extend Leu-19-positive cell processes towards to the unstained tumor cells. Furthermore, the Leu-19 antigen was identified on physiological tissues, especially on all neuroendocrine cells analyzed. The cross-reactions observed with Leu-19 mAb were confirmed using mAb NKH-1, which is also directed against the Leu-19 molecule. Thus, the expression of Leu-19 on neuroectodermal cells is another example of antigen sharing between specialized immune cells and the nervous system. Springer-Verlag, 1990 Natural killer cells nationallicence Immunohistochemistry nationallicence Leu-19 antigen nationallicence Brain tumors nationallicence Neuroendocrine cells nationallicence Nawrotzki R. Institut für Genetik, Universität Köln, Weyertal 121, D-5000, Köln 41, Federal Republic of Germany aut Starzinski-Powitz A. Institut für Genetik, Universität Köln, Weyertal 121, D-5000, Köln 41, Federal Republic of Germany aut Weber F. Abtellung für Neurochirurgie, Städtisches Krankenhaus, Ostmerheimer Strasse 200, D-5000, Köln 91, Federal Republic of Germany aut Schröder R. Labor für Neuropathologie, Institut für Pathologie, Universität Köln, Joseph-Stelzmann-Strasse 9, D-5000, Köln 41, Federal Republic of Germany aut Acta Neuropathologica Springer-Verlag 79/5(1990-02-01), 520-527 0001-6322 79:5<520 1990 79 401 https://doi.org/10.1007/BF00296112 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00296112 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nawrotzki R. Institut für Genetik, Universität Köln, Weyertal 121, D-5000, Köln 41, Federal Republic of Germany aut NATIONALLICENCE 700 1- Starzinski-Powitz A. Institut für Genetik, Universität Köln, Weyertal 121, D-5000, Köln 41, Federal Republic of Germany aut NATIONALLICENCE 700 1- Weber F. Abtellung für Neurochirurgie, Städtisches Krankenhaus, Ostmerheimer Strasse 200, D-5000, Köln 91, Federal Republic of Germany aut NATIONALLICENCE 700 1- Schröder R. Labor für Neuropathologie, Institut für Pathologie, Universität Köln, Joseph-Stelzmann-Strasse 9, D-5000, Köln 41, Federal Republic of Germany aut NATIONALLICENCE 773 0- Acta Neuropathologica Springer-Verlag 79/5(1990-02-01), 520-527 0001-6322 79:5<520 1990 79 401 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer